Sickle cell disease serum induces NADPH enzyme subunit expression and oxidant production in leukocytes
Sickle cell disease serum induces NADPH enzyme subunit expression and oxidant production in leukocytes
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DOI：
10.1179/102453310x12719010991786
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发表时间：
2010-12-01
期刊：
影响因子：
1.9
通讯作者：
Conran, Nicola
中科院分区：
文献类型：
--
作者：
Almeida, Camila B.;Franco-Penteado, Carla;Conran, Nicola
氧化应激在镰状细胞病（SCD）中起重要作用，导致溶血、血管闭塞过程和内皮功能障碍。为了研究SCD个体的血清对血细胞的氧化状态的影响，将来自对照个体、稳态SCD患者和接受羟基脲治疗（SCDHU）的SCD患者的血清合并，并观察它们对从健康个体分离的中性粒细胞中的氧化应激和损伤的标志物的影响。用SCD血清（10%v/v; 2小时）孵育对照中性粒细胞，而不是血小板或红细胞，显著增加了它们的活性氧（ROS）产生。SCD血清孵育中性粒细胞中ROS产生增加与超氧阴离子产生增加、细胞凋亡和烟酰胺腺嘌呤二核苷酸磷酸氧化酶亚基表达增加相关。虽然SCDHU个体的血清也诱导中性粒细胞产生ROS，但其氧化能力似乎较低。结果表明，SCD个体的血清中的因素有助于ROS的产生和白细胞的氧化损伤。
Oxidative stress plays a significant role in sickle cell disease (SCD), contributing to haemolysis, vaso-occlusive processes and endothelial dysfunction. To study the effects that the serum of SCD individuals has on the oxidative state of blood cells, sera were pooled from control individuals, steady-state SCD patients and SCD patients on hydroxyurea therapy (SCDHU), and their effects on markers of oxidative stress and damage in neutrophils isolated from healthy individuals observed. Incubation of control neutrophils, but not platelets nor red blood cells, with SCD serum (10% v/v; 2 hours) significantly augmented their production of reactive oxygen species (ROS). Increased ROS production in SCD serum-incubated neutrophils was associated with increased superoxide anion generation, apoptosis and increased nicotinamide adenine dinucleotide phosphate oxidase subunit expression. Although serum from SCDHU individuals also induced ROS generation in neutrophils, its oxidative capacity appeared to be lower. Results suggest that factors in the serum of SCD individuals contribute to ROS generation and oxidative damage in leukocytes.