CHANGES OF THE CORNEA ENDOTHELIUM AFTER ULTRAVIOLET RADIATION
CHANGES OF THE CORNEA ENDOTHELIUM AFTER ULTRAVIOLET RADIATION
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紫外线照射后角膜内皮的变化
DOI：
10.1111/j.1755-3768.1982.tb05780.x
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发表时间：
1982
影响因子：
3.4
通讯作者：
E. G. Olsen
中科院分区：
文献类型：
--
作者：
A. Ringvold;M. Davanger;E. G. Olsen
用市售紫外灯照射后，用角膜内皮和扫描电子显微镜研究了兔角膜内皮。将动物放置在距离辐射源50 cm处，大多数实验的暴露时间为20 min。在角膜内皮显微镜下，内皮显示出许多外切无反射区域，这些区域在照射后4天至8个月内观察到。扫描电镜观察到多种变化。暴露后一天，细胞显示出模糊的轮廓，它们膨胀到前房。在某些区域，图案主要由标记的凹槽优先沿着细胞分界线，而其他区域显示粗糙表面的细胞，因为细胞质突起和颗粒状沉积物。在第3天阶段，存在明显的多形性，并且一些细胞正在被排斥的途中。一周后，内皮的鹅卵石外观即将下降。部分排斥细胞仍然很常见。此外，明显的，环形的局部压痕出现模仿前面描述的凹槽。这种现象沿着一些单个内皮细胞布满微绒毛是损伤后8个月的异常表现。结果表明，尽管紫外线在角膜中被大量吸收，但角膜内皮损伤的阈值剂量并不超过实际使用的阈值剂量。令人惊讶的是，内皮对重复辐射损伤具有抵抗力。
The rabbit cornea endothelium has been studied with specular and scanning electron microscopy after exposure with a commercially available ultraviolet lamp. The animals were plased 50 cm from the source, and most experiments were run with a 20 min exposure time. In the specular microscope, the endothelium revealed numerous circumscript reflex‐free areas, which were observed from 4 days up to 8 months after the irradiation. A wide varity of changes were found in the scanning electron microscope. One day after the exposure the cells showed indistinct outlines, and they were bulging into the anterior chamber. In some regions the pattern was dominated by marked grooves preferentially along the cell demarcations, whereas other regions showed rough surfaced cells because of cytoplasmic projections and grainy deposits. At the 3 days stage there was a marked pleomorphy, and some cells were on their way to being rejected. After one week the cobblestone appearance of the endothelium was about to decline. Partly rejected cells were still common. In addition, distinct, ringshaped local impressions occurred imitating the previous described grooves. This phenomenon along with some single endothelial cells studded with microvilli were the abnormal findings 8 months after the damage. It is concluded that although ultraviolet radiation is largely absorbed in the cornea, the threshold dose for damage of the endothelium is not beyond those used for practical purpose. The endothelium was surprisingly resistent towards repeated radiation damage.