Fat-1 transgenic mice: A new model for omega-3 research
Fat-1 transgenic mice: A new model for omega-3 research
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DOI：
10.1016/j.plefa.2007.10.010
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发表时间：
2007-11-01
影响因子：
3
通讯作者：
Kang, Jing X.
中科院分区：
文献类型：
--
作者：
Kang, Jing X.
一个适当的动物模型，可以消除混杂因素的饮食将是非常有帮助的评价营养素，如n-3脂肪酸的健康效应。我们最近产生了一个脂肪-1转基因小鼠表达秀丽隐杆线虫脂肪-1基因编码的n-3脂肪酸去饱和酶，转换n-6到n-3脂肪酸（这是不存在的哺乳动物）。fat-1转基因小鼠能够从n-6型产生n-3脂肪酸，导致其器官和组织中n-6脂肪酸水平降低的n-3脂肪酸丰富，而不需要饮食n-3供应。给转基因和野生型同窝仔喂食相同的饮食（高n-6）可在这些动物中产生不同的脂肪酸谱。因此，该模型允许进行良好对照的研究，而不受饮食的潜在混杂因素的干扰。转基因小鼠现在被广泛使用，并正在成为研究n-3脂肪酸的益处及其作用的分子机制的新工具。(C)2007爱思唯尔有限公司保留所有权利。
An appropriate animal model that can eliminate confounding factors of diet would be very helpful for evaluation of the health effects of nutrients such as n-3 fatty acids. We recently generated a fat-1 transgenic mouse expressing the Caenorhabditis elegans fat-1 gene encoding an n-3 fatty acid desaturase that converts n-6 to n-3 fatty acids (which is absent in mammals). The fat-1 transgenic mice are capable of producing n-3 fatty acids from the n-6 type, leading to abundant n-3 fatty acids with reduced levels of n-6 fatty acids in their organs and tissues, without the need of a dietary n-3 supply. Feeding an identical diet (high in n-6) to the transgenic and wild-type littermates can produce different fatty acid profiles in these animals. Thus, this model allows well-controlled studies to be performed, without the interference of the potential confounding factors of diet. The transgenic mice are now being used widely and are emerging as a new tool for studying the benefits of n-3 fatty acids and the molecular mechanisms of their action. (C) 2007 Elsevier Ltd. All rights reserved.