The Entire Nucleotide Sequence of Friend-Related and Paralysis-Inducing PVC-441 Murine Leukemia Virus (MuLV) and Its Comparison with Those of PVC-211 MuLV and Friend MuLV
The Entire Nucleotide Sequence of Friend-Related and Paralysis-Inducing PVC-441 Murine Leukemia Virus (MuLV) and Its Comparison with Those of PVC-211 MuLV and Friend MuLV
复制标题
Friend相关麻痹性PVC-441鼠白血病病毒(MuLV)的全核苷酸序列及其与PVC-211 MuLV和Friend MuLV的比较
作者：
A. Tanaka;K. Oka;Keiji Tanaka;A. Jinno;S. Ruscetti;K. Kai
摘要：PVC-441小鼠白血病病毒（MuLV）是Friend MuLV （F-MuLV）衍生的神经致病性逆转录病毒PVC群中的一员。为了确定PVC-441与先前鉴定的PVC-211 MuLV神经致病性差异的分子基础，我们测定了PVC-441 MuLV的全核苷酸序列，并与PVC-211和F-MuLV进行了比较。结果表明，PVC-441和PVC-211 mulv是由F-MuLV的随机突变形成的，并且发育不同。PVC-441和PVC-211在其分子克隆再生的病毒中保持了不同的致病性，导致病理差异的序列可能定位于env基因。根据PVC-441的核苷酸序列推断出其氨基酸序列与PVC-211存在许多差异，其中最显著的是两种病毒中SU蛋白的129位存在差异。对脑毛细血管内皮细胞（RTEC-6）和中国仓鼠卵巢细胞（CHO-K1）的宿主范围研究表明，PVC-441与PVC-211一样可以感染这些细胞，但其感染效率低于PVC-211。这些结果可能解释了PVC-441和PVC-211在神经致病性上的差异。
ABSTRACT PVC-441 murine leukemia virus (MuLV) is a member of the PVC group of Friend MuLV (F-MuLV)-derived neuropathogenic retroviruses. In order to determine the molecular basis for the difference in neuropathogenicity between PVC-441 and the previously characterized PVC-211 MuLVs, the entire nucleotide sequence of PVC-441 MuLV was determined and compared with those of PVC-211 and F-MuLV. The results suggest that PVC-441 and PVC-211 MuLVs were formed as a result of random mutations of F-MuLV and developed differently. The distinct pathogenicities of PVC-441 and PVC-211 MuLVs were maintained in the viruses regenerated from their molecular clones, and the sequences responsible for the pathological differences observed can be localized to the env gene. The amino acid sequence of PVC-441 deduced from its nucleotide sequence revealed a number of differences from PVC-211, the most striking of which was a difference at position 129 of the SU proteins in the two viruses. Host range studies with a brain capillary endothelial cell line (RTEC-6) and Chinese hamster ovary cells (CHO-K1) revealed that PVC-441, like PVC-211, could infect these cells but its efficiency of infection was lower than that of PVC-211. These results may account for the difference in neuropathogenicity between PVC-441 and PVC-211.
弗兰德鼠白血病病毒神经致病变种的细胞趋向性和在啮齿动物神经系统中的定位。
DOI：
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发表时间：
1992
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
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作者：
Hoffman,PM;Cimino,EF;Robbins,DS;Broadwell,RD;Powers,JM;Ruscetti,SK
通讯作者：
Ruscetti,SK