Biological and chemical determination of dioxin-like compounds in sediments by means of a sediment triad approach in the catchment area of the River Neckar
Biological and chemical determination of dioxin-like compounds in sediments by means of a sediment triad approach in the catchment area of the River Neckar
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DOI：
10.1023/a:1020549103898
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发表时间：
2002-10-01
期刊：
影响因子：
2.7
通讯作者：
Braunbeck, T
中科院分区：
文献类型：
--
作者：
Hollert, H;Dürr, M;Braunbeck, T
为了评估内卡河集水区选定地点的沉积物质量，采用综合评估方法评估二恶英类沉积物化合物的潜在生态危害。该方法是基于7-乙氧基试卤灵-O-脱乙基酶（EROD）诱导鸡胚肝培养和多环芳烃（根据美国环境保护署的优先多环芳烃）的综合化学分析。大多数沉积物提取物作为EROD诱导剂表现出高效力。在一个受污水处理厂影响的沉积物样本中，可以确定非常高的浓度，即930纳克生物测定2，3，7，8-四氯二苯并对二恶英（TCDD）当量（生物TEQs）/克有机碳。然而，在没有一个样品中，超过6%的EROD诱导效力可以解释的多环芳烃化学分析。因此，浸提液中存在具有EROD诱导效力的未分析化合物。根据pH值的沉积物样品的分馏允许本地化的EROD诱导化合物的主要部分在中性馏分。然而，EROD诱导的一个重要部分也可以解释的酸性馏分。根据Chapman提出的沉积物质量三元组概念，对大型底栖动物的原地变化进行了研究。EROD测定和化学分析预测的结果与原位变化的比较，通过测量的污染指数和生态毒理学指数根据Carmargo，揭示了高生态相关性的主要网站的生物测定和化学分析的结果。
To evaluate the sediment quality of selected sites in the catchment area of the River Neckar, an integrative assessment approach was used to assess the ecological hazard potential of dioxin-like sediment compounds. The approach is based on 7-ethoxyresorufin-O-deethylase (EROD) induction in embryonic chicken liver culture and comprehensive chemical analyses of polycyclic aromatic hydrocarbons (priority PAHs according to the US Environmental Protection Agency). The majority of the sediment extracts exhibited high potencies as EROD-inducers. In one sediment sample, which was influenced by a sewage treatment plant, a very high concentration of 930 ng bioassay 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalents (bio-TEQs)/g organic carbon could be determined. However, in none of the samples, more than 6% of the EROD-inducing potency could be explained by the PAHs analyzed chemically. Thus, non-analyzed compounds with EROD-inducing potency were present in the extracts. A fractionation of sediment samples according to pH allowed to localize the major part of EROD-inducing compounds in the neutral fractions. However, a significant portion of the EROD induction could also be explained by the acidic fractions. Following the concept of the Sediment Quality Triad according to Chapman, in situ alterations of macrozoobenthos were examined. A comparison of the results predicted by the EROD assay and chemical analyses with alterations in situ, as measured by means of the saprobic index and the ecotoxicological index according to Carmargo, revealed a high ecological relevance of the results of bioassays and chemical analyses for major sites.