Gene expression profiling in the post-mortem human brain - no cause for dismay
Gene expression profiling in the post-mortem human brain - no cause for dismay
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DOI：
10.1016/s0891-0618(01)00099-0
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发表时间：
2001-07-01
影响因子：
2.8
通讯作者：
Emson, PC
中科院分区：
文献类型：
--
作者：
Bahn, S;Augood, S;Emson, PC
全球表达谱技术，如微阵列技术，有望给生物学带来革命性的变化。很快，就有可能研究整个人类基因组转录水平上的变化。人们对这些技术最终将阐明复杂的神经精神障碍，如精神分裂症的病理过程抱有很大希望。这些科学进步反过来又重新点燃了人们对死后脑组织的巨大兴趣和需求。高质量的死后组织无疑是利用分子图谱技术研究复杂脑部疾病的基本前提。在这篇综述中，我们发现死后的脑组织可以产生高质量的mRNA和完整的蛋白质抗原，这使得传统的分子生物学方法和新的图谱技术的成功应用成为可能。我们还考虑在身体组织上使用激光捕获显微解剖。这项最近开发的技术使实验者能够在单细胞水平上探索细胞功能的分子基础。激光捕获显微解剖与高通量剖析技术的结合为获得单个神经元的精确遗传指纹提供了机会，从而可以比较正常和病理状态。(C)2001 Elsevier Science B.V.保留所有权利。
Global expression profiling techniques such as microarray technology promise to revolutionize biology. Soon it will be possible to investigate alterations at the transcript level of the entire human genome. There is great hope that these techniques will at last shed light on the pathological processes involved in complex neuropsychiatric disorders such as schizophrenia. These scientific advances in turn have re-kindled a great interest and demand for post-mortem brain tissue. Good quality post-mortem tissue undoubtedly is the fundamental prerequisite to investigate complex brain disorders with Molecular profiling techniques. In this review we show that post-mortem brain tissue can yield good quality mRNA and intact protein antigens which allow the successful application of traditional molecular biology methods as well as novel profiling techniques. We also consider the use of laser-capture microdissection on post-mortem tissue. This recently developed technique allows the experimenter to explore the molecular basis of cellular function at the single cell level. The combination of laser-capture microdissection with high throughput profiling techniques offers opportunities to obtain precise genetic fingerprints of individual neurons allowing comparisons of normal and pathological states. (C) 2001 Elsevier Science B.V. All rights reserved.