Dietary aluminosilicate supplement enhances immune activity in mice and reinforces clearance of porcine circovirus type 2 in experimentally infected pigs
Dietary aluminosilicate supplement enhances immune activity in mice and reinforces clearance of porcine circovirus type 2 in experimentally infected pigs
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DOI：
10.1016/j.vetmic.2009.11.009
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发表时间：
2010-07-14
影响因子：
3.3
通讯作者：
Lee, Bong-Joo
中科院分区：
文献类型：
--
作者：
Jung, Bock-Gie;Toan, Nguyen Tat;Lee, Bong-Joo
铝硅酸盐是沸石、膨润土、斜发沸石等粘土矿物的主要成分。矿物质具有许多有益的活性，特别是在调节免疫系统方面。本研究的目的是评估饮食铝硅酸盐补充剂（DAS）在小鼠中的免疫增强作用，并证明DAS对实验感染猪的猪圆环病毒2型（PCV 2）的清除作用，作为开发用于猪的抗生素替代品的第一步。与对照组相比，DAS组小鼠IFN-γ、IL-4和TNF-α的相对mRNA表达水平、多形核白细胞吞噬活性、血清抗体产生水平和脾脏B细胞比率均显著升高（每个喂养组3个重复，每个重复5只小鼠）。结果表明，DAS能增强小鼠的细胞免疫和体液免疫。在实验PCV 2感染的猪中，与感染后28天的对照组相比，DAS组猪的鼻拭子、血清和肺中的病毒基因组载量显著降低（每组3头猪）。相应的组织病理学分析表明，与对照组相比，DAS组中的猪显示出轻度和不太严重的异常变化，表明DAS加强了实验感染猪中PCV 2的清除。这可能与DAS在小鼠中的一般免疫增强作用有关。因此，DAS将有助于动物，特别是猪的健康。(C)2009爱思唯尔有限公司版权所有。
Aluminosilicate is the major component of clay minerals such as zeolite, bentonite and clinoptilolite. The minerals possess a number of beneficial activities, especially in regulating the immune system. The aims of the present study were to evaluate immune enhancing effects of dietary aluminosilicate supplement (DAS) in mice, and to demonstrate clearance effects of DAS against porcine circovirus type 2 (PCV2) in experimentally infected pigs as an initial step towards the development of an antibiotic substitute for use in pigs. Relative messenger RNA expression levels of interferon-gamma, interleukin-4 and tumor necrosis factor-alpha, phagocytic activities of polymorphonuclear leucocytes, serum antibody production level and spleen B cell ratio were significantly increased in the DAS groups of mice compared with the control group (each feeding group had three replications with 5 mice each). The results indicated that general immune activity including cellular and humoral immunity could be enhanced by DAS in mice. In experimentally PCV2-infected pigs, the load of viral genome in nasal swab, serum and lung of the DAS group of pigs was significantly decreased compared with the control group at 28 days post-infection (each group three pigs). Corresponding histopathological analyses demonstrated that pigs in the DAS group displayed mild and less severe abnormal changes compared with the control group, indicating that DAS reinforces clearance of PCV2 in experimentally infected pigs. This may relate to general immune enhancing effects of DAS in mice. Therefore DAS will help the health of animal, especially in swine. (C) 2009 Elsevier B.V. All rights reserved.