Impact of farnesol and Corsodyl® on Candida albicans forming dual biofilm with Streptococcus mutans
Impact of farnesol and Corsodyl® on Candida albicans forming dual biofilm with Streptococcus mutans
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DOI：
10.1111/odi.12873
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发表时间：
2018-09-01
期刊：
影响因子：
3.8
通讯作者：
Bujdakova, H.
中科院分区：
文献类型：
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作者：
Cernakova, L.;Jordao, L.;Bujdakova, H.
本工作研究了白念珠菌和变形链球菌在羟基磷灰石表面上形成的生物膜后，暴露于群体感应分子法尼醇（200 M）与稀释的漱口水Corsodyl（（R））（0.0001%氯己定二葡萄糖酸盐）的比较。材料和方法法尼醇的细胞毒性通过Galleria mellonella存活测定来评估。通过集落形成单位测定暴露于法尼醇和Corsodyl（R）后生物膜细胞的活力。双物种生物膜的形态和结构进行了评估，通过扫描电子显微镜。ResultsFarnesol没有表现出对幼虫生存的毒性作用。虽然200 M法尼醇有效地减少了酵母菌丝过渡的双重生物膜，它并没有影响变形链球菌的生长。此外，尽管存在法尼醇，但观察到许多芽生孢子。Corsodyl（（R））减少了双生物膜中的变形链球菌，但对白色念珠菌没有影响。ConclusionThis study showed，200M法尼醇调制了具有变形链球菌的双物种生物膜中的白色念珠菌，但对变形链球菌没有表现出抗菌活性。此外，似乎变形链球菌提供了支持白色念珠菌的酵母形式生长的条件。漱口水Corsodyl（（R））可减少变形链球菌，但对白色念珠菌无效。
ObjectiveThis work studied the biofilm formed by Candida albicans and Streptococcus mutans on a hydroxyapatite surface after exposure to the quorum-sensing molecule farnesol (200M) in comparison with the diluted mouthwash Corsodyl((R)) (0.0001% chlorhexidine digluconate).Materials and MethodsThe cytotoxicity of farnesol was evaluated by Galleria mellonella surviving assay. The viability of biofilm cells after exposure to farnesol and Corsodyl((R)) was determined by colony-forming units. The morphology and structure of a dual-species biofilm was evaluated by scanning electron microscopy.ResultsFarnesol did not exhibit a toxic effect on larval survival. While 200M farnesol effectively reduced the yeast-to-hyphae transition in the dual biofilm, it did not affect the growth of S.mutans. Additionally, despite the presence of farnesol, many blastospores were observed. Corsodyl((R)) reduced S.mutans in the dual biofilm, but did not influence C.albicans.ConclusionThis study showed that 200M farnesol modulated C.albicans in a dual-species biofilm with S.mutans, but did not exhibit antimicrobial activity against S.mutans. Moreover, it seems that S.mutans provides conditions that support the growth of the yeast form of C.albicans. The mouthwash Corsodyl((R)) reduces S.mutans, but was not effective against C.albicans.