Expression of multiple alpha 2-adrenergic receptor messenger RNA species in rat tissues.
Expression of multiple alpha 2-adrenergic receptor messenger RNA species in rat tissues.
复制标题
多种 α2-肾上腺素能受体信使 RNA 种类在大鼠组织中的表达。
DOI：
10.1161/01.hyp.15.6.881
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Gavras,H
中科院分区：
文献类型：
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作者：
Flordellis,CS;Castellano,M;Franco,R;Zannis,VI;Gavras,H
我们用人血小板α2-肾上腺素能受体探针研究了大鼠α2-肾上腺素能受体的组织分布和信使RNA形式。在严格的杂交和洗涤条件下，我们检测到3.8kb的mRNA种类。这种形式的丰度遵循脾、肾、脑干和皮质、骨骼肌和肺的顺序，与已报道的阿尔法2受体活性的丰度和组织分布一致。在不严格的杂交条件下，大脑皮层和脑干中也检测到了3.0kb的mRNA，肾脏中也检测到了4.1kb的mRNA。3.0kb形式的组织分布不同于α1和β肾上腺素能受体以及D2多巴胺能受体。对大鼠和人基因组DNA的mRNA分析结合Southern杂交分析表明：1)除了3.8kb的大鼠α2-肾上腺素能受体转录本外，大鼠中还有其他形式的mRNA不对应于先前描述的肾上腺素能受体mRNA物种；2)大鼠中不止一个α2-肾上腺素能受体基因以组织特异性的方式表达。
We used a human platelet alpha 2-adrenergic receptor probe to study the tissue distribution and messenger RNA (mRNA) forms of the rat alpha 2-adrenergic receptor. Under stringent conditions of hybridization and washing, we detected an mRNA species of 3.8 kb. The abundance of this form follows the order spleen, kidney, brain stem and cortex, and skeletal muscle and lung and is consistent with the reported abundance and tissue distribution of the alpha 2 receptor activity. A 3.0 kb mRNA form was also detected in cerebral cortex and brain stem and a 4.1 kb mRNA form was observed in kidney under less stringent hybridization conditions. The tissue distribution of the 3.0 kb form is different from that of alpha 1- and beta-adrenergic receptors and the D2 dopaminergic receptor. The mRNA analysis combined with Southern blot analysis of rat and human genomic DNA indicate that: 1) in addition to a 3.8 kb rat alpha 2-adrenergic receptor transcript, there are other mRNA forms in the rat that do not correspond to previously described adrenergic receptor mRNA species and 2) more than one alpha 2-adrenergic receptor gene in the rat is expressed in a tissue-specific manner.