Lon Protease Has Multifaceted Biological Functions in Acinetobacter baumannii
Lon Protease Has Multifaceted Biological Functions in Acinetobacter baumannii
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DOI：
10.1128/jb.00536-18
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发表时间：
2019-01-01
影响因子：
3.2
通讯作者：
Godoy, Veronica G.
中科院分区：
文献类型：
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作者：
Ching, Carly;Yang, Brendan;Godoy, Veronica G.
鲍曼不动杆菌是一种革兰氏阴性机会致病菌，已知可以在恶劣的环境条件下生存，并且是医院获得性感染的主要原因。具体来说，鲍曼不动杆菌的多细胞群落（称为生物膜）可以承受干燥并在医院表面和设备上存活。生物膜是嵌入由蛋白质、糖和/或 DNA 组成的自身产生的细胞外基质中的细菌。生物膜中的细菌受到保护，免受包括抗生素在内的环境压力的影响，这为细菌提供了生存的选择优势。尽管已知一些基因产物在鲍曼不动杆菌的这一发育过程中发挥作用，但其机制和信号传导仍然大多未知。在这里，我们发现鲍曼不动杆菌中的 Lon 蛋白酶影响生物膜的发育，并具有其他重要的生理作用，包括运动和细胞包膜。 Lon 蛋白酶存在于生命的各个领域，参与调节过程并维持细胞稳态。这些数据揭示了 Lon 蛋白酶在影响鲍曼不动杆菌抵抗应激和维持活力的关键过程中的重要性。重要性鲍曼不动杆菌是一种机会性病原体，是医院获得性感染的主要原因。鲍曼不动杆菌很难根除和管理，因为众所周知，这种细菌能够在干燥条件下坚强生存并迅速获得抗生素耐药性。我们试图研究鲍曼不动杆菌中生物膜的形成，因为关于这种细菌中生物膜的形成还有很多未知之处。生物膜是多细胞细菌群落，附着在表面，很难从医院设备和植入装置中清除。我们的研究确定了鲍曼不动杆菌中保守的细菌蛋白酶 Lon 的多方面生理作用。这些作用包括生物膜形成、运动性和活力。这项工作广泛影响并扩大了对鲍曼不动杆菌生物学的理解，这将使我们能够找到消灭这种细菌的有效方法。
Acinetobacter baumannii is a Gram-negative opportunistic pathogen that is known to survive harsh environmental conditions and is a leading cause of hospital-acquired infections. Specifically, multicellular communities (known as biofilms) of A. baumannii can withstand desiccation and survive on hospital surfaces and equipment. Biofilms are bacteria embedded in a self-produced extracellular matrix composed of proteins, sugars, and/or DNA. Bacteria in a biofilm are protected from environmental stresses, including antibiotics, which provides the bacteria with selective advantage for survival. Although some gene products are known to play roles in this developmental process in A. baumannii, mechanisms and signaling remain mostly unknown. Here, we find that Lon protease in A. baumannii affects biofilm development and has other important physiological roles, including motility and the cell envelope. Lon proteases are found in all domains of life, participating in regulatory processes and maintaining cellular homeostasis. These data reveal the importance of Lon protease in influencing key A. baumannii processes to survive stress and to maintain viability.IMPORTANCE Acinetobacter baumannii is an opportunistic pathogen and is a leading cause of hospital-acquired infections. A. baumannii is difficult to eradicate and to manage, because this bacterium is known to robustly survive desiccation and to quickly gain antibiotic resistance. We sought to investigate biofilm formation in A. baumannii, since much remains unknown about biofilm formation in this bacterium. Biofilms, which are multicellular communities of bacteria, are surface attached and difficult to eliminate from hospital equipment and implanted devices. Our research identifies multifaceted physiological roles for the conserved bacterial protease Lon in A. baumannii. These roles include biofilm formation, motility, and viability. This work broadly affects and expands understanding of the biology of A. baumannii, which will permit us to find effective ways to eliminate the bacterium.