Lipid-cell interactions in human monocytes investigated by doubly-resonant coherent anti-Stokes Raman scattering microscopy
Lipid-cell interactions in human monocytes investigated by doubly-resonant coherent anti-Stokes Raman scattering microscopy
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DOI：
10.1117/1.3544585
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发表时间：
2011-02-01
影响因子：
3.5
通讯作者：
Huser, Thomas
中科院分区：
文献类型：
--
作者：
Weeks, Tyler;Schie, Iwan;Huser, Thomas
我们证明，双共振相干反斯托克斯拉曼散射可以提供增强的和高度特定的对比度的分子含有独特的拉曼活性小分子基团。这种组合为难以通过拉曼光谱法区分的分子提供了对比度。在此，将人单核细胞与氘代油酸或17-十八炔酸（具有末端乙炔基的脂肪酸）一起孵育。氘代脂肪酸的碳-氘伸缩振动以及含炔脂肪酸的独特炔伸缩振动用于为这些外源性游离脂肪酸提供对比。这些独特的模式与所有脂肪酸固有的常见脂肪族碳氢伸缩振动的组合允许对这些独特分子进行双共振检测，并使我们能够在细胞内每个分子共振本身不产生足够信号的区域中检测这些脂质的存在。(C)2011年，美国光电仪器工程师学会（SPIE）。[DOI：10.1117/1.3544585]
We demonstrate that doubly-resonant coherent anti-Stokes Raman scattering can provide enhanced and highly specific contrast for molecules containing unique Raman-active small molecular groups. This combination provides contrast for molecules that can otherwise be difficult to discriminate by Raman spectroscopy. Here, human monocytes were incubated with either deuterated oleic acid or 17-octadecynoic acid (a fatty acid with an end terminal acetylene group). The carbon-deuterium stretching vibration of the deuterated fatty acid, as well as the unique alkyne stretching vibration of the alkyne-containing fatty acid, were used to provide contrast for these exogenous free fatty acids. The combination of these unique modes with the common aliphatic carbon-hydrogen stretching vibration inherent to all fatty acid allowed for doubly-resonant detection of these unique molecules and enabled us to detect the presence of these lipids in areas within a cell where each molecular resonance by itself did not generate sufficient signal. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI:10.1117/1.3544585]