Background-dependentWolbachia-mediated insecticide resistance inLaodelphax striatellus
Background-dependentWolbachia-mediated insecticide resistance inLaodelphax striatellus
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灰飞虱背景依赖性沃尔巴克氏体介导的杀虫剂抗性
DOI：
10.1111/1462-2920.14974
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发表时间：
2020-07-01
影响因子：
5.1
通讯作者：
Guo, Huifang
中科院分区：
文献类型：
--
作者：
Li, Yongteng;Liu, Xiangdong;Guo, Huifang
尽管现在已知兼性内共生体可以保护昆虫宿主免受病原体和寄生物的侵害，但内共生体对杀虫剂抗性的影响仍不清楚。在这里，我们表明，在某些遗传背景下，沃尔巴克氏体与小褐飞虱（Laodelphax striatellus）对常用杀虫剂噻嗪酮的抗性增强有关，而在其他背景下则没有影响。在中国云南省楚雄市建立的来自实验性噻嗪酮抗性菌株的三个沃尔巴克氏体感染品系和来自对噻嗪酮敏感品系的一个品系中，去除沃尔巴克氏体后，对噻嗪酮的敏感性增加。江苏省如皋市野外种群建立的沃尔巴克氏体感染品系的易感性也明显增加。然而，来自中国江苏省南京市和奉贤市的两个野外种群没有明显增加。当沃尔巴克氏体渗入不同的遗传背景，然后去除沃尔巴克氏体时，数据表明沃尔巴克氏体效应取决于核背景以及沃尔巴克氏体菌株。然而，沃尔巴克氏体密度与共生体相关的噻嗪酮耐药成分之间没有关系。结果表明，沃尔巴克氏体与耐化学性相关的影响是复杂且不可预测的，但也可能是巨大的。
Although facultative endosymbionts are now known to protect insect hosts against pathogens and parasitoids, the effects of endosymbionts on insecticide resistance are still unclear. Here we show thatWolbachiaare associated with increased resistance to the commonly used insecticide, buprofezin, in the small brown planthopper (Laodelphax striatellus) in some genetic backgrounds while having no effect in other backgrounds. In threeWolbachia-infected lines from experimental buprofezin-resistant strains and one line from a buprofezin-susceptible line established from Chuxiong, Yunnan province, China, susceptibility to buprofezin increased after removal ofWolbachia. An increase in susceptibility was also evident in aWolbachia-infected line established from a field population in Rugao, Jiangsu province. However, no increase was evident in two field populations from Nanjing and Fengxian, Jiangsu province, China. WhenWolbachiawas introgressed into different genetic backgrounds, followed byWolbachiaremoval, the data pointed toWolbachiaeffects that depend on the nuclear background as well as on theWolbachiastrain. However, there was no relationship betweenWolbachiadensity and the component of buprofezin resistance associated with the symbiont. The results suggest thatWolbachiaeffects associated with chemical resistance are complex and unpredictable, but also that they can be substantial.