Sexual dimorphism of the fifth component of mouse complement.
Sexual dimorphism of the fifth component of mouse complement.
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DOI：
10.1084/jem.160.2.411
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发表时间：
1984-08-01
期刊：
影响因子：
--
通讯作者：
Tamura N
中科院分区：
文献类型：
--
作者：
Baba A;Fujita T;Tamura N
小鼠补体成分5（C5）的性二态性是通过去唾液酸乙二胺四乙酸（EDTA）-血浆在琼脂糖凝胶上的等电聚焦，然后用抗小鼠C5进行免疫固定来检测的。雄性血浆显示两条C5带，碱性和酸性，而雌性血浆只有一条碱性C5带。除C5缺陷株外，所有供试小鼠品系均获得了相同的二态性模式。碱性和酸性C5在抗原性和溶血性上彼此无法区分。在两种性别的新生小鼠中，观察到相同水平的碱性C5，但仅轻微检测到酸性C5。向小鼠注射睾酮导致雌性小鼠出现酸性C5，而雄性小鼠则增加。相比之下，注射雌二醇降低了酸性C5。这些结果表明，酸性C5的表达是在睾酮的控制下。
Sexual dimorphism of mouse complement component 5 (C5) was detected by isoelectric focusing of desialated ethylenediamine tetraacetic acid (EDTA)-plasma on agarose gel, followed by immunofixation with anti- mouse C5. Male plasma displayed two C5 bands, basic and acidic, while female plasma had only a basic C5 band. In all mouse strains tested except C5-deficient strains, the identical patterns of the dimorphism were obtained. The basic and acidic C5 were antigenically and hemolytically indistinguishable from each other. In the neonatal mice of both sexes, the basic C5 was observed at the same levels, but the acidic C5 was only slightly detected. Injection of testosterone into mice resulted in an appearance of the acidic C5 in females and its increase in males. By contrast, injection of estradiol decreased the acidic C5. These results indicate that the expression of the acidic C5 is under the control of testosterone.