Sublethal Effects of Triflumezopyrim on Biological Traits and Detoxification Enzyme Activities in the Small Brown Planthopper Laodelphax striatellus (Hemiptera: Delphacidae)
Sublethal Effects of Triflumezopyrim on Biological Traits and Detoxification Enzyme Activities in the Small Brown Planthopper Laodelphax striatellus (Hemiptera: Delphacidae)
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DOI：
10.3389/fphys.2020.00261
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发表时间：
2020-04
影响因子：
4
通讯作者：
Shuirong Zhang;Xuegui Wang;Fuchuan Gu;Changwei Gong;Lin Chen;Yuming Zhang;A. Hasnain;Li-tao Shen;Chunxian Jiang
中科院分区：
文献类型：
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作者：
Shuirong Zhang;Xuegui Wang;Fuchuan Gu;Changwei Gong;Lin Chen;Yuming Zhang;A. Hasnain;Li-tao Shen;Chunxian Jiang
褐飞虱[Laodelphax striatellus(Fallén)(半翅目，飞蝇科)]是水稻上最具破坏性的害虫之一，对多种化学杀虫剂产生了很强的抗性。Triflumezopyrim是Corteva Agiscience(原杜邦作物保护公司)开发的一种新型中离子杀虫剂，具有有效的生物活性，可控制飞虱等吸食性昆虫。然而，关于曲氟咪唑对纹纹夜蛾生长和繁殖的影响尚未见报道。在本研究中，通过对一株敏感的纹纹夜蛾菌株(F0代)进行5轮药剂筛选，获得了F5代。采用年龄阶段生命表法，研究了亚致死浓度(LC_(50))的三氟唑啉对纹纹夜蛾生物学参数的影响。与F0代相比，F5代的内禀增长率(R)、有限增长率(λ)和净繁殖率(R0)显著降低，但对平均寿命(T)无显著影响。解毒酶活性测定结果表明，F5代的谷胱甘肽S转移酶和细胞色素P450单加氧酶活性显著高于F0代。结果表明，F5代的VG和VGR含量均显著低于F0代。此外，我们还检测了蜕皮激素受体(ECR)、VG和VGR在F0和F5代中的相对表达，发现F5代雌成虫VG和VGR的相对表达水平明显低于F0代(P<0.05)。基于这些结果，亚致死浓度(半数致死浓度，LC_(50))的三氟唑啉可能会抑制纹纹夜蛾的世代生长和繁殖。此外，我们的研究结果可能为进一步研究亚致死剂量的氟甲嘧啶对纹枯病菌的适应性和抗性机制提供参考。
The small brown planthopper [Laodelphax striatellus (Fallén) (Hemiptera, Delphacidae)] is one of the most destructive insect pests of rice and has developed strong resistance to several kinds of chemical insecticides. Triflumezopyrim, a novel mesoionic insecticide developed by Corteva Agriscience (formerly DuPont Crop Protection), has efficient biological activity in controlling sucking insects, such as the planthopper. However, the effects of triflumezopyrim on the growth and reproduction of L. striatellus have not been reported. In this study, an F5 generation was obtained by conducting five rounds of insecticide screening on a sensitive L. striatellus strain (F0 generation). An age-stage life table procedure was used to evaluate the effects of a sublethal concentration (LC50) of triflumezopyrim on the biological parameters of L. striatellus. Compared with those of the F0 generation, the intrinsic rate of increase (r), the finite rate (λ), and the net reproductive rate (R0) of the F5 generation were significantly decreased; nevertheless, the average duration of life (T) was not significantly affected. The results of detoxification enzyme activity assays indicated that the glutathione S-transferase and cytochrome P450 monooxygenase (P450) activities in the F5 generation were significantly higher than those in the F0 generation. The contents of vitellogenin (Vg) and vitellogenin receptor (VgR) were also detected, and the results indicated that the contents of Vg and VgR in the F5 generation were significantly decreased compared to those in the F0 generation. Furthermore, we detected the relative expression of ecdysone receptor (EcR), Vg, and VgR in the F0 and F5 generations and found that the relative expression levels of Vg and VgR in the F5 generation female adults were obviously lower than those in the F0 generation (P 0.05). Based on these results, a sublethal concentration (median lethal concentration, LC50) of triflumezopyrim may inhibit the generational growth and reproduction of L. striatellus. Moreover, our results may provide a reference for further studies of the suitability and resistance mechanisms of L. striatellus subjected to a sublethal dose of triflumezopyrim.