Long-term consumption of dried bonito dashi (a traditional Japanese fish stock) reduces anxiety and modifies central amino acid levels in rats
Long-term consumption of dried bonito dashi (a traditional Japanese fish stock) reduces anxiety and modifies central amino acid levels in rats
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DOI：
10.1179/1476830514y.0000000124
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发表时间：
2015-08-01
影响因子：
3.6
通讯作者：
Furuse, Mitsuhiro
中科院分区：
文献类型：
--
作者：
Funatsu, Shoichiro;Kondoh, Takashi;Furuse, Mitsuhiro
干鲣鱼是日本传统的鱼汤，因其独特的风味而增强了各种菜肴的适口性。每日摄入dashi也被证明可以改善人类的情绪状态，如紧张焦虑。本研究旨在探讨大石对大鼠焦虑/抑郁样行为的有益影响以及脑和血浆中氨基酸水平的变化。雄性Wistar大鼠长期（29天;实验1）或单次口服给药（实验2）给予干鲣鱼或水。焦虑和抑郁样行为分别采用旷场和强迫游泳试验进行测试。在海马、下丘脑、小脑和颈静脉中测量氨基酸浓度。在长期（29天）的消费，大鼠给予2%的大石频繁进入中心区，并花费更多的时间相比，在开放领域的测试中的水控制。然而，dashi对抑郁样行为无效。在海马中，羟脯氨酸，鹅丝氨酸，缬氨酸的浓度增加，而天冬酰胺和苯丙氨酸的浓度降低。在下丘脑中，蛋氨酸浓度降低。在单次口服实验中，1%、2%和10%的大石对行为没有影响。仅在海马中的α-氨基己二酸、胱硫醚和鸟氨酸浓度中观察到显著性。干鲣鱼是一种具有抗焦虑作用的功能性食品。每天摄入达什，即使是在烹饪中发现的较低浓度，也会减少焦虑并改变大脑中的氨基酸水平。
Dried bonito dashi, a traditional Japanese fish stock, enhances palatability of various dishes because of its specific flavor. Daily intake of dashi has also been shown to improve mood status such as tension-anxiety in humans. This study aimed at investigating beneficial effects of dashi ingestion on anxiety/depression-like behaviors and changes in amino acid levels in the brain and plasma in rats. Male Wistar rats were given either dried bonito dashi or water for long-term (29 days; Experiment 1) or single oral administration (Experiment 2). Anxiety and depression-like behaviors were tested using the open field and forced swimming tests, respectively. Concentrations of amino acids were measured in the hippocampus, hypothalamus, cerebellum, and jugular vein. During the long-term (29 days) consumption, rats given 2% dashi frequently entered the center zone and spent more time compared with the water controls in the open field test. However, the dashi was ineffective on depression-like behavior. In the hippocampus, concentrations of hydroxyproline, anserine, and valine were increased by dashi while those of asparagine and phenylalanine were decreased. In the hypothalamus, the methionine concentration was decreased. In a single oral administration experiment, the dashi (1%, 2% or 10%) showed no effects on behaviors. Significance was observed only in the concentrations of alpha-aminoadipic acid, cystathionine, and ornithine in the hippocampus. Dried bonito dashi is a functional food having anxiolytic-like effects. Daily ingestion of the dashi, even at lower concentrations found in the cuisine, reduces anxiety and alters amino acid levels in the brain.