POLY(ADP-RIBOSE) POLYMERASE FORMS LOOPS WITH DNA
POLY(ADP-RIBOSE) POLYMERASE FORMS LOOPS WITH DNA
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DOI：
10.1016/s0006-291x(87)80219-x
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发表时间：
1987-11-13
影响因子：
3.1
通讯作者：
DEMURCIA, G
中科院分区：
文献类型：
--
作者：
GRADWOHL, G;MAZEN, A;DEMURCIA, G
用凝胶延迟电泳法和电子显微镜研究了高纯度的小牛胸腺多聚腺苷二磷酸核糖聚合酶与不同拓扑形式的pBR322 DNA的相互作用。我们发现：(I)在DNA上没有缺口的情况下，酶与超螺旋(形式I)DNA具有显著的亲和力，(Ii)在单链断裂存在的情况下，聚(ADP-核糖)聚合酶优先结合到形式II，(Iii)在所有情况下，酶分子经常位于DNA交叉处，(Iv)酶与DNA发生协同结合。
The interaction between highly purified poly(ADP-ribose) polymerase from calf thymus and different topological forms of pBR322 DNA has been studied by gel retardation electrophoresis and electron microscopy. We show that: (i) in the absence of nicks on DNA the enzyme has a marked affinity for supercoiled (form I) DNA, (ii) in the presence of single stranded breaks poly(ADP-ribose) polymerase preferentially binds to form II, (iii) in all cases enzyme molecules are frequently located at DNA intersections, (iv) a cooperative binding of the enzyme on DNA occurs.