Binding of N-linked bovine fetuin glycopeptides to isolated rabbit hepatocytes: Gal/GalNAc hepatic lectin discrimination between Gal beta(1,4)GlcNAc and Gal beta(1,3)GlcNAc in a triantennary structure.
Binding of N-linked bovine fetuin glycopeptides to isolated rabbit hepatocytes: Gal/GalNAc hepatic lectin discrimination between Gal beta(1,4)GlcNAc and Gal beta(1,3)GlcNAc in a triantennary structure.
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N-连接牛胎球蛋白糖肽与分离的兔肝细胞的结合：三触角结构中 Gal/GalNAc 肝凝集素区分 Gal beta(1,4)GlcNAc 和 Gal beta(1,3)GlcNAc。
DOI：
10.1021/bi00367a055
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Lee,YC
中科院分区：
文献类型：
--
作者：
Townsend,RR;Hardy,MR;Wong,TC;Lee,YC
约翰霍普金斯大学生物学系和麦科勒姆-普拉特研究所，巴尔的摩，马里兰州21218接收于1986年3月7日;修订后的Mandarin pt接收于1986年5月14日摘要：用链霉蛋白酶、氨肽酶M和羧肽酶Y消化后，从牛胎球蛋白中分离出糖肽。用叔丁氧羰基酪氨酸对糖肽进行衍生化，然后用反相高效液相色谱法进行分离。使用400-MHz 1H NMR，发现在三个N-连接的糖基化位点中的每一个处的无唾液酸三触角寡糖是以下两种结构的组合，其中第三分支是Gal/3（1，3）GlcNAc或Gal/3（1，3）GlcNAc：
Department of Biology and McCollum-Pratt Institute, The Johns Hopkins University, Baltimore, Maryland 21218 Received March 7, 1986; Revised Manuscript Received May 14, 1986 abstract: Glycopeptides were isolated from bovine fetuin after digestion with Pronase, aminopeptidase M, and carboxypeptidase Y. Theglycopeptides were derivatized with tert-butyloxycarbonyltyrosine and separated on the basis of peptide by using reverse-phase high-performanceliquid chromatography. Using 400-MHz'H NMR, the asialotriantennary oligosaccharides at each of the three N-linked glycosylation sites were found to be combinations of the following two structures in which the third branch is either Gal (3 (l, 4) GlcNAc or Gal/3 (l, 3) GlcNAc: