Comparison of metabolic adaptation and biofilm formation of Actinobacillus pleuropneumoniae field isolates from the upper and lower respiratory tract of swine with respiratory disease
Comparison of metabolic adaptation and biofilm formation of Actinobacillus pleuropneumoniae field isolates from the upper and lower respiratory tract of swine with respiratory disease
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DOI：
10.1016/j.vetmic.2019.108532
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发表时间：
2020-01-01
影响因子：
3.3
通讯作者：
Hennig-Pauka, Isabel
中科院分区：
文献类型：
--
作者：
Aper, Doris;Froembling, Janna;Hennig-Pauka, Isabel
大多数疾病暴发是由放线杆菌（A.）胸膜肺炎是由已经在扁桃体组织中预先定殖的猪引起的，在扁桃体组织中病原体被保护免于暴露于用于治疗的抗生素物质。正如最近在实验条件下所表明的那样，A.胸膜肺炎显示宿主组织特异性代谢适应。在本研究中，对A.从患有胸膜肺炎的急性临床症状并显示特征性病理性肺改变的20头猪的肺以及扁桃体和鼻组织中回收胸膜肺炎田间分离株。32 A对猪下呼吸道和上呼吸道的代谢适应。使用傅立叶变换红外（FTIR）光谱作为高分辨率代谢指纹法检查胸膜肺炎血清型2田间分离株。所有菌株均表现出对器官组织的代谢适应，这通过FTIR光谱的分层聚类分析反映，与先前在实验条件下观察到的相似。值得注意的是，在微量滴定板测定中，发现了来自同一动物不同组织的分离物的抗微生物剂抗性模式和最小抑制浓度的差异，但没有发现生物膜产生能力的差异。胸膜肺炎田间分离株通常能够形成生物膜，尽管是以血清型特异性方式而不是器官特异性方式形成。A.胸膜肺炎血清型6分离株形成的生物膜显著多于其它血清型。此外，生物膜的产生与肺部病变评分呈负相关，扁桃体分离株往往比肺部分离株更容易受到具有高生物利用度的抗菌物质的影响。
Most outbreaks of disease due to infection with Actinobacillus (A.) pleuropneumoniae are caused by pigs already pre-colonised in tonsillar tissue, where the pathogen is protected from exposure to antibiotic substances administered for treatment. As it has been shown recently under experimental conditions, A. pleuropneumoniae displays host tissue-specific metabolic adaptation. In this study, pairs of A. pleuropneumoniae field isolates were recovered from lung as well as from tonsillar and nasal tissue from 20 pigs suffering from acute clinical signs of pleuropneumonia and showing characteristic pathological lung alterations. Metabolic adaptation to the porcine lower and upper respiratory tract of 32 A. pleuropneumoniae serotype 2 field isolates was examined using Fourier transform infrared (FTIR) spectroscopy as a high resolution metabolic fingerprinting method. All strains showed metabolic adaptations to organ tissue reflected by hierarchical cluster analysis of FTIR spectra similar to those previously observed under experimental conditions. Notably, differences in antimicrobial resistance patterns and minimal inhibitory concentrations of isolates from different tissues in the same animal, but not in biofilm production capability in a microtiter plate assay were found. Overall, biofilm formation was observed for 71 % of the isolates, confirming that A. pleuropneumoniae field isolates are generally able to form biofilms, although rather in a serotype-specific than in an organ-specific manner. A. pleuropneumoniae serotype 6 isolates formed significantly more biofilm than the other serotypes. Furthermore, biofilm production was negatively correlated to the lung lesion scores and tonsillar isolates tended to be more susceptible to antimicrobial substances with high bioavailability than lung isolates.