Genetic requirements for inheritance of RNAi in C-elegans
Genetic requirements for inheritance of RNAi in C-elegans
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DOI：
10.1126/science.287.5462.2494
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发表时间：
2000-03-31
期刊：
影响因子：
56.9
通讯作者：
Mello, CC
中科院分区：
文献类型：
--
作者：
Grishok, A;Tabara, H;Mello, CC
在秀丽线虫中，双链RNA的引入触发了序列特异性通用干扰(RNAi)，并将其传递给后代。研究了与这一现象相关的遗传特性。干扰效应的传递是通过显性的外源因子实现的。RNAi途径基因rde-1和rde-4的野生型活性对该干扰剂的形成是必需的，但在以后的干扰中不需要。相反，rde-2和mut-7基因需要在下游进行干扰。这些发现为基因外序列特异性沉默因子的种系传递提供了证据，并暗示rde-1和rde-4与遗传剂的形成有关。
In Caenorhabditis elegans, the introduction of double-stranded RNA Triggers sequence-specific generic interference (RNAi) that is transmitted to offspring. The inheritance properties associated with this phenomenon were examined. Transmission of the interference effect occurred through a dominant extragenic agent. The wild-type activities of the RNAi pathway genes rde-1 and rde-4 were required for the formation of this interfering agent but were not needed for interference thereafter. In contrast, the rde-2 and mut-7 genes were required downstream for interference. These findings provide evidence for germ line transmission of an extragenic sequence-specific silencing factor and implicate rde-1 and rde-4 in the formation of the inherited agent.