Loss of intervening sequences in genomic mouse α-globin DNA inserted in an infectious retrovirus vector
Loss of intervening sequences in genomic mouse α-globin DNA inserted in an infectious retrovirus vector
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DOI：
10.1038/299265a0
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发表时间：
1982-09
期刊：
影响因子：
64.8
通讯作者：
K. Shimotohno;H. Temin
中科院分区：
文献类型：
--
作者：
K. Shimotohno;H. Temin
在一些高致癌性逆转录病毒（一些病毒致癌基因）和正常细胞基因组（cDNA基因、假基因或加工基因）中发现了缺乏在其他明显同源基因中存在的干预序列的基因1-10。这种cDNA基因可能是由同源基因的剪接（中间序列被移除）RNA转录物的反转录而产生的。具有中间序列的基因失去这些序列的另一种可能的方式是在逆转录病毒载体的复制过程中。为了验证这一假设，我们将含有两个中间序列的小鼠基因组α-珠蛋白DNA插入到感染性逆转录病毒载体的DNA中，并在这里报道了在病毒复制过程中从子代病毒的DNA中去除中间序列。
Genes lacking the intervening sequences that are present in otherwise apparently homologous genes have been found in some highly oncogenic retroviruses (some viral oncogenes) and in the normal cell genome (cDNA genes, pseudogenes or processed genes)1–10. Such cDNA genes might have arisen by reverse transcription of the spliced (intervening sequences removed) RNA transcript of the homologous genes. Another possible way for genes with intervening sequences to lose such sequences would be during replication in a retrovirus vector. To test this hypothesis we inserted genomic mouseα-globin DNA containing two intervening sequences11into the DNA of an infectious retrovirus vector12and report here that the intervening sequences were removed from DNA of progeny virus during virus replication.