BASAL BODY MOVEMENTS AS A MECHANISM FOR MITOCHONDRIAL GENOME SEGREGATION IN THE TRYPANOSOME CELL-CYCLE
BASAL BODY MOVEMENTS AS A MECHANISM FOR MITOCHONDRIAL GENOME SEGREGATION IN THE TRYPANOSOME CELL-CYCLE
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DOI：
10.1038/352731a0
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发表时间：
1991-08-22
期刊：
影响因子：
64.8
通讯作者：
GULL, K
中科院分区：
文献类型：
--
作者：
ROBINSON, DR;GULL, K
布鲁氏锥虫的线粒体基因组以环状DNA分子的复杂链链网络的形式组织。这一团DNA，被称为着丝体，存在于单个线粒体的一个独特位置，并在细胞周期的一个离散的、周期性的S期进行复制。着丝体的单拷贝特性表明，存在一种机制，确保了复制副本对每个子细胞的分离保真度。从历史上看，关于这一机制的性质的推测通常归因于着丝体和鞭毛基体之间的密切联系。我们在这里提供了直接的证据，证明线粒体DNA复合体确实与基底体相连，并且在细胞周期中，着丝体DNA的分离依赖于微管介导的新旧鞭毛基底体的分离。这个独特的系统可能代表了进化上古老的基因组分离机制的残余。
THE mitochondrial genome of Trypanosoma brucei is organized in the form of a complex catenated network of circular DNA molecules. This mass of DNA, known as the kinetoplast, is present at a unique site in the single mitochondrion, and is replicated in a discrete, periodic S phase of the cell cycle. The single-copy nature of the kinetoplast suggests that there is a mechanism ensuring segregation fidelity of replicated copies to each daughter cell. Historically, speculation regarding the nature of this mechanism has often attributed significance to the close association between the kinetoplast and the flagellum basal body. We provide here direct evidence that this mitochondrial DNA complex is indeed linked to the basal body, and segregation of the kinetoplast DNA is dependent on a microtubule-mediated separation of the new and old flagellar basal bodies during the cell cycle. This unique system may represent the remnants of an evolutionarily archaic mechanism for genome segregation.