Mechanisms of patulin toxicity under conditions that inhibit yeast growth
Mechanisms of patulin toxicity under conditions that inhibit yeast growth
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DOI：
10.1021/jf052264g
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发表时间：
2006-03-08
影响因子：
6.1
通讯作者：
Iwahashi, H
中科院分区：
文献类型：
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作者：
Iwahashi, Y;Hosoda, H;Iwahashi, H
棒曲霉素，4-羟基- 4h -呋喃[3,2c]吡喃-2(6H)- 1，是在农产品中发现的特性最好、传播最广泛的真菌毒素之一。然而，展霉素引起毒性的机制尚不清楚。因此，通过分析酵母在展霉素刺激下的转录组来表征展霉素的细胞毒性。有趣的是，棒曲霉素诱导的酵母基因表达谱被发现与抗真菌农用化学品硫脲（thiuram）、马耐（maneb）和锌（zineb）处理后获得的基因表达谱相似。此外，还发现展霉素可以激活蛋白质降解，特别是蛋白酶体活性、硫氨基酸代谢和氧化应激防御系统。烷基化对DNA的损伤也被提出，这似乎是通过重组和切除修复机制修复的。此外，该结果为农产品中展霉素的检测提供了潜在的生物标记基因。这一结果为利用酵母转录组系统评价化学物质，特别是难以通过有机合成获得的天然化学物质提供了可能性。
Patulin, 4-hydroxy-4H-furo[3,2c]pyran-2(6H)-one, is one of the best characterized and most widely disseminated mycotoxins found in agricultural products. Nonetheless, the mechanisms by which patulin causes toxicity are not well understood. Thus, the cytotoxicity of patulin was characterized by analysis of the yeast transcriptome upon challenge with patulin. Interestingly, patulin-induced yeast gene expression profiles were found to be similar to gene expression patterns obtained after treatment with the antifungal agricultural chemicals thiuram, maneb, and zineb. Moreover, patulin treatment was found to activate protein degradation, especially proteasome activities, sulfur amino acid metabolism, and the defense system for oxidative stress. Damage to DNA by alkylation was also suggested, and this seemed to be repaired by recombinational and excision repair mechanisms. Furthermore, the results provide potential biomarker genes for the detection of patulin in agricultural products. The results suggest the possibility of applying the yeast transcriptome system for the evaluation of chemicals, especially for natural chemicals that are difficult to get by organic synthesis.