Development of paratransgenic Artemia as a platform for control of infectious diseases in shrimp mariculture.
Development of paratransgenic Artemia as a platform for control of infectious diseases in shrimp mariculture.
复制标题
开发副转基因卤虫作为控制虾海水养殖传染病的平台。
DOI：
10.1111/j.1365-2672.2009.04479.x
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发表时间：
2010
影响因子：
4
通讯作者：
Durvasula,R
中科院分区：
文献类型：
--
作者：
Subhadra,B;Hurwitz,I;Fieck,A;Rao,DVS;SubbaRao,G;Durvasula,R
目的：研究卤虫体内重组蛋白的积累和保留，以优化对虾副转基因疾病的防治。方法和结果：将表达荧光标记蛋白的转基因大肠杆菌和表达功能性鼠源单链抗体DB 3的转基因蓝藻聚球藻分别饲喂卤虫。几种标记分子（例如GFP、DS Red和DB 3）在用E.大肠杆菌在卤虫的肠道内是明显的。S.表达DB 3的芽孢杆菌在卤虫肠道内积累，通过ELISA检测到8-10 h的抗体活性，与转基因S的最高密度的时间段一致。结论：青蒿素诱导的转基因细菌或藻类在10 h内积累了重组蛋白，这些重组蛋白仍具有生物学活性。研究的意义和影响：通过常用的饲料生物如Artemia，表达针对虾中海水养殖感染因子的分子可能为正在进行的全球努力提供强大的新工具，以增加粮食供应。
Aim:To study the accumulation and retention of recombinant proteins inArtemiagut for optimizing paratransgenic disease control in shrimp aquaculture.Methods and Results:TransgenicEscherichia coliexpressing fluorescent marker proteins and the transgenic cyanobacteriumSynechococcus bacillarusexpressing a functional murine single chain antibody, DB3, were fed toArtemia franciscana. Stable expression and retention of several marker molecules (e.g. GFP, DS Red and DB3) up to 10 h after of feeding withE. coliwere evident within the gut ofArtemia. Engineered strains ofS. bacillarusexpressing DB3 accumulated within the gut ofArtemiawith detectable antibody activity for 8–10 h of feeding via ELISA, coincident with the time period of the highest density of transgenicS. bacillarusin theArtemiagut.Conclusions:Artemiafed transgenic bacteria or algae accumulated recombinant proteins for up to 10 h that retained biological activity. Co‐delivery of multiple recombinant proteins simultaneously in the gut ofArtemiawas also demonstrated.Significance and Impact of the Study:Expression of molecules that target infectious agents of mariculture in shrimp via commonly deployed feed organisms such asArtemiacould potentially offer powerful new tools in the ongoing global effort to increase food supply.