A suicidal DNA vaccine co-expressing two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus antigens induce protective responses
A suicidal DNA vaccine co-expressing two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus antigens induce protective responses
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共表达猪繁殖与呼吸综合征病毒抗原的两种主要膜相关蛋白的自杀性 DNA 疫苗可诱导保护性反应
DOI：
10.1007/s10529-008-9905-0
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发表时间：
2009-04-01
影响因子：
2.7
通讯作者：
Chen, Huanchun
中科院分区：
文献类型：
--
作者：
Jiang, Yunbo;Fang, Liurong;Chen, Huanchun
构建了共表达猪繁殖与呼吸综合征病毒（PRRSV）GP 5 m和M蛋白的自杀性DNA疫苗pSFV-ORF 5 m/ORF 6。在小鼠免疫中，自杀性DNA疫苗pSFV-ORF 5 m/ORF 6可诱导特异性免疫应答。在病毒攻击前用pSFV-ORF 5 m/ORF 6免疫仔猪，然后评价免疫原性和保护效率：产生PRRSV特异性中和抗体和淋巴细胞增殖反应。在PRRSV攻击后，这些免疫应答进一步加强，并获得部分保护。
We constructed a suicidal DNA vaccine pSFV-ORF5m/ORF6 co-expressing GP5m (a modified GP5) and M proteins of porcine reproductive and respiratory syndrome virus (PRRSV). In mice immunization, specific immune responses were elicited by the suicidal DNA vaccine pSFV-ORF5m/ORF6. The immunogenicity and protective efficiency was then evaluated in piglets immunized with pSFV-ORF5m/ORF6 before virus challenge: PRRSV-specific neutralizing antibodies and lymphocyte proliferative responses were developed. Post-PRRSV challenge, these immune responses were further boosted and partial protection was obtained.