In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region
In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region
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DOI：
10.1016/s1074-7613(00)80463-x
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发表时间：
1998-01-01
期刊：
影响因子：
32.4
通讯作者：
Miyajima, A
中科院分区：
文献类型：
--
作者：
Mukouyama, YS;Hara, T;Miyajima, A
造血干细胞(HSCs)的起源及其对生长因子的需求还知之甚少。在这里，我们描述了一种新的主动脉-性腺-中肾(AGM)区的体外培养系统，在那里首先会出现长期再生的HSC。我们证明了OSM在AGM中表达，并且是体外扩增多潜能造血祖细胞所必需的。此外，OSM还能促进内皮细胞簇的形成。因此，OSM似乎是AGM中多潜能造血祖细胞发育的关键细胞因子，可能作用于HSC和内皮细胞之间的共同前体细胞。利用巨噬细胞集落刺激因子(M-CSF)缺乏的OP/OP突变胚胎的AG-M培养，我们还显示了M-CSF在胎儿骨髓生成中的关键作用。
The origin of hematopoietic stem cells (HSCs) and their growth factor requirement are poorly understood. Here we describe a new in vitro culture system of the aorta-gonad-mesonephros (AGM) region, where long-term repopulating HSCs first arise. We demonstrate that oncostatin M (OSM) is expressed in the AGM and is absolutely required for the expansion of multipotential hematopoietic progenitors in vitro. In addition, OSM enhances the formation of endothelial cell clusters. Thus, OSM appears to be a key cytokine for the development of multipotential hematopoietic progenitors in the AGM, possibly acting on common precursor cells between HSCs and endothelial cells. fly using the AG M culture derived from macrophage colony-stimulating factor (M-CSF)-deficient op/op mutant embryos, we also show a pivotal role for M-CSF in fetal myelopoiesis.