SYNTHESIS AND USE OF A NOVEL N-FORMYL PEPTIDE DERIVATIVE TO ISOLATE A HUMAN N-FORMYL PEPTIDE RECEPTOR CDNA
SYNTHESIS AND USE OF A NOVEL N-FORMYL PEPTIDE DERIVATIVE TO ISOLATE A HUMAN N-FORMYL PEPTIDE RECEPTOR CDNA
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DOI：
10.1016/0006-291x(90)91143-g
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发表时间：
1990-05-16
影响因子：
3.1
通讯作者：
VIGNAIS, P
中科院分区：
文献类型：
--
作者：
BOULAY, F;TARDIF, M;VIGNAIS, P
N-甲酰甲硫基多肽是一种强大的趋化物质，能与中性粒细胞质膜上的特定受体结合。在COS细胞表达载体CDM8中构建了HL-60细胞分化为对N-甲酰甲硫基多肽高度敏感的粒细胞的cDNA文库。分离到一个能与新型高效亲水性N-甲酰-甲硫氨酸-亮氨酸-苯丙氨酸衍生物结合的克隆。转基因COS细胞呈现两类结合位点，Kd值分别为0.5~1 nM和5~10 nM。该基因全长1.9kb，开放阅读框1050bp，编码350个残基蛋白。疏水图分析显示了7个疏水片段，其模式与G蛋白偶联受体非常相似。
N-formyl-methionyl peptides are powerful chemoattractants which bind to specific receptors on the neutrophil plasma membrane. A cDNA library from HL-60 cells, differentiated into granulocytes highly responsive to N-formyl-methionyl peptides, was constructed in the COS cell expression vector CDM8. A cDNA clone was isolated that conferred to COS cells the ability to bind a new and highly efficient hydrophilic derivative of N-formyl-Met-Leu-Phe-Lys. The transfected COS cells displayed two classes of binding sites with Kd values of 0.5-1 nM and 5-10 nM, respectively. The cDNA was 1.9 kb long with a 1050 bp open reading frame encoding a 350 residue protein. The hydropathy plot analysis revealed seven hydrophobic segments, a pattern quite similar to that of G protein-coupled receptors.