Detection and quantification of human immunodeficiency virus RNA in patient serum by use of the polymerase chain reaction.
Detection and quantification of human immunodeficiency virus RNA in patient serum by use of the polymerase chain reaction.
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使用聚合酶链反应检测和定量患者血清中的人类免疫缺陷病毒 RNA。
DOI：
10.1093/infdis/163.4.862
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Merigan,TC
中科院分区：
文献类型：
--
作者：
Holodniy,M;Katzenstein,DA;Sengupta,S;Wang,AM;Casipit,C;Schwartz,DH;Konrad,M;Groves,E;Merigan,TC
应用聚合酶链反应（PeR）和非同位素酶联亲和试验检测和定量HIV阳性个体血清中的HIV RNA。在55名未接受治疗的HIV感染者中，19名无症状者中有9名、16名艾滋病相关复合体（ARC）患者中有11名和20名艾滋病患者中有18名检测到血清HIV RNA，根据吸光度和已知gag基因RNA拷贝数之间的关系，拷贝数范围为102至105 × 104/200 µl血清。线性回归分析显示，与供体外周血单个核细胞（PBMC）共培养的42例患者血清的感染滴度与PCR产物吸光度之间存在相关性（r=.70，P> .01）。PCR检测的血清HIV RNA也与血清p24抗原阳性、CD 4计数<400/mm 3和HIV相关症状或疾病的存在相关。通过PCR定量无细胞血清中的感染性HIV RNA可用作疾病进展或监测抗病毒治疗的标志物。
Human immunodeficiency virus (HIV) RNA was detected and quantified in the serum of HIVseropositive individuals using the polymerase chain reaction (PeR) and a nonisotopic enzymelinked affinity assay. Of 55 HIV-infected patients who were not receiving therapy, serum HIV RNA was detected in 9 of 19 who were asymptomatic, 11 of 16 with AIDS-related complex (ARC), and 18of 20 with AIDS, with copy numbers ranging from 102to ⩾5 × 104/200 µl of serum based on a relationship between absorbance and known copy number ofgaggene RNA. Linear regression analysis demonstrated a correlation between infectious titer in 42 patient sera cocultured with donor peripheral blood mononuclear cells (PBMC) and PCR product absorbance (r= .70,P> .01). Serum HIV RNA detected by PCR also correlated with serum p24 antigen positivity, CD4 counts <400/mm3, and the presence of HIV-related symptoms or disease. Quantification of infectious HIV RNA in cell-free serum by PCR may be useful as a marker for disease progression or in monitoring antiviral therapy.