A study on the activity of fibroblast cells in connection with tissue recovery in the wounds of skin injury after whole-body irradiation.
A study on the activity of fibroblast cells in connection with tissue recovery in the wounds of skin injury after whole-body irradiation.
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DOI：
10.1269/jrr.45.341
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发表时间：
2004-06
影响因子：
2
通讯作者：
Jifu Qu;T. Cheng;C. Shi;Yuan Lin;X. Ran
中科院分区：
文献类型：
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作者：
Jifu Qu;T. Cheng;C. Shi;Yuan Lin;X. Ran
6 Gy的伽玛射线全身照射（WBI）会导致受伤皮肤组织恢复受损，并延迟愈合过程。为了了解WBI是否对伤口成纤维细胞有损伤作用，我们分离培养了伤口合并WBI和单纯切口的成纤维细胞，并通过直接细胞计数、增殖细胞核抗原（PCNA）免疫组化染色和TUNEL测定了与组织修复相关的增殖、附着、粘附和凋亡能力。结果表明，创伤后第3天和第5天，伤口合并WBI成纤维细胞的增殖能力和附着、粘附能力明显低于单纯切口组，而伤口合并WBI成纤维细胞的凋亡率明显升高。这些数据表明，WBI可能对伤口成纤维细胞产生损伤作用，这可能是WBI合并伤口愈合受损的主要原因之一。
The 6 Gy of whole-body irradiation (WBI) with gamma rays results in an impairment of injured skin tissue recovery and renders a delay in the healing process. For an understanding of whether WBI has damaging effects on fibroblasts in wounds, fibroblasts in wounds combined with WBI and those of simple incision were isolated and cultivated, and abilities connected with tissue repair, including proliferation, attachment, adhesion, and apoptosis, were determined by direct cell count, immunohistochemical staining for proliferation cell nuclear antigen (PCNA), and TUNEL assay. The results showed that the abilities of proliferation and the attachment and adhesion of fibroblasts from wounds combined with WBI significantly decreased in comparison with those having simple incisions on the 3rd and 5th days of posttrauma, whereas the apoptotic ratio of fibroblasts from wounds combined with WBI significantly increased. These data suggest that WBI may exert damaging effects on fibroblasts in wounds, which might be one of the dominant reasons for the impaired healing of wounds combined with WBI.