Gene silencing by adenovirus-delivered siRNA
Gene silencing by adenovirus-delivered siRNA
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DOI：
10.1016/s0014-5793(03)00209-6
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发表时间：
2003-03-27
期刊：
影响因子：
3.5
通讯作者：
Reske, SN
中科院分区：
文献类型：
--
作者：
Shen, CX;Buck, AK;Reske, SN
RNA干扰是由21-23核苷酸短干扰RNA （siRNA）介导的双链RNA诱导同源mRNA同源性依赖性降解的过程。在这里，我们描述了一种简单的病毒载体，利用定义明确的HI-RNA启动子和常规腺病毒，将siRNA有效地递送到哺乳动物细胞中。在这项初步研究中，p53被该载体靶向。我们的研究结果表明，在乳腺癌MCF-7和肺癌A549细胞中，p53被有效和特异性地敲除，这表明这种表达siRNA的重组腺病毒系统在功能基因组学、癌症基因治疗和病毒抑制方面的应用前景广阔。(C) 2003年由Elsevier Science B.V.代表欧洲生化学会联合会出版。
RNA interference is the process that double-stranded RNA induces the homology-dependent degradation of cognate mRNA mediated by 21-23 nucleotide short interfering RNA (siRNA). Here, we describe a simple virus vector for efficient delivery of siRNA into mammalian cells utilizing the well-defined HI-RNA promoter and conventional adenovirus. In this pilot study, p53 was targeted by this vector. Our results demonstrate efficient and specific knock-down of p53 in breast cancer MCF-7 and lung carcinoma A549 cells and indicate a prospective application of this siRNA expressing recombinant adenovirus system in functional genomics, cancer gene therapy and virus inhibition. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.