Modulation of transcriptional corepressor activity of prospero‐related homeobox protein (Prox1) by SUMO modification
Modulation of transcriptional corepressor activity of prospero‐related homeobox protein (Prox1) by SUMO modification
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DOI：
10.1016/j.febslet.2008.09.057
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发表时间：
2008-11
期刊：
影响因子：
3.5
通讯作者：
Shi-Fang Shan;Lin-fang Wang;Jianwei Zhai;Yi Qin;Hua Ouyang;Y. Kong;Jing Liu;Y. Wang;Youhua Xie
中科院分区：
文献类型：
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作者：
Shi-Fang Shan;Lin-fang Wang;Jianwei Zhai;Yi Qin;Hua Ouyang;Y. Kong;Jing Liu;Y. Wang;Youhua Xie
Prospero 相关同源盒蛋白 (Prox1) 在许多组织和器官的发育中发挥着重要作用。在本研究中，我们证明 Prox1 在培养细胞中被小泛素样蛋白 SUMO-1 修饰。突变分析确定了 Prox1 抑制域内至少四个潜在的 sumoylation 位点。我们的数据表明 Prox1 的 sumoylation 减少了其与 HDAC3 的相互作用，从而下调了其辅阻遏物活性。这些发现表明，SUMO 化可能作为调节 Prox1 辅阻遏物活性的新机制。结构摘要：
Prospero-related homeobox protein (Prox1) plays essential roles in the development of many tissues and organs. In the present study, we show that Prox1 is modified by the small ubiquitin-like protein SUMO-1 in cultured cells. Mutation analysis identified at least four potential sumoylation sites within the repression domain of Prox1. Our data indicate that sumoylation of Prox1 reduces its interaction with HDAC3 and as a result downregulates its corepressor activity. These findings suggest that sumoylation may serve as a novel mechanism for the regulation of Prox1’s corepressor activity. STRUCTURED SUMMARY: