Release of immunoreactive somatostatin from hypothalamic cells in culture: inhibition by gamma-aminobutyric acid.
Release of immunoreactive somatostatin from hypothalamic cells in culture: inhibition by gamma-aminobutyric acid.
复制标题
从培养的下丘脑细胞中释放免疫反应性生长抑素：γ-氨基丁酸的抑制。
DOI：
10.1073/pnas.77.9.5552
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发表时间：
1980
影响因子：
11.1
通讯作者：
Leeman,SE
中科院分区：
文献类型：
--
作者：
Gamse,R;Vaccaro,DE;Gamse,G;DiPace,M;Fox,TO;Leeman,SE
制备胎鼠分散的下丘脑细胞原代培养物，研究免疫反应性生长抑素的释放。这些培养物的生长抑素免疫活性在接种后的前2周内增加，并在之后的几周内很容易被测量到；凝胶渗透和反相色谱对这种材料进行了表征。用60 mM K+或藜芦碱去极化后，免疫活性生长抑素的释放呈钙依赖性，并与合成的生长抑素在反相色谱上进行共层析。河豚毒素可阻断藜芦碱引起的释放。然而，即使在没有外源刺激的情况下，细胞也会将免疫反应活跃的生长抑素释放到培养液中。超过70%的这种紧张性释放是钙依赖的，并被河豚毒素抑制，这表明培养物中的自发电活动导致了免疫反应性生长抑素的释放。γ-氨基丁酸抑制免疫活性生长抑素的紧张性释放，荷包牡丹碱可逆转这一作用。这些发现支持了伽马氨基丁酸在体内抑制生长抑素释放的假设。
Primary cultures of dispersed hypothalamic cells were prepared from embryonic rats to study the release of immunoreactive somatostatin. The immunoreactive somatostatin content of these cultures increased during the first 2 weeks after plating and was readily measurable for several weeks thereafter; this material was characterized by gel permeation and reverse-phase chromatography. Depolarization of the cells with 60 mM K+ or with veratridine resulted in a calcium-dependent release of immunoreactive somatostatin which cochromatographed with synthetic somatostatin on reverse-phase chromatography. Tetrodotoxin blocked the veratridine-evoked release. However, even in the absence of exogenous stimuli, immunoreactive somatostatin was released by the cells into the medium. More than 70% of this tonic release was found to be calcium dependent and to be inhibited by tetrodotoxin, indicating that spontaneous electrical activity in the cultures leads to a release of immunoreactive somatostatin. gamma-Aminobutyric acid inhibited the tonic release of immunoreactive somatostatin and this was reversed by bicuculline. These findings support the hypothesis that gamma-aminobutyric acid inhibits somatostatin release in vivo.