Evidence for in vivo internalization of human leukocyte elastase by alveolar macrophages.
Evidence for in vivo internalization of human leukocyte elastase by alveolar macrophages.
复制标题
肺泡巨噬细胞体内人白细胞弹性蛋白酶内化的证据。
DOI：
10.1164/arrd.1982.125.6.779
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Campbell,E
中科院分区：
文献类型：
--
作者：
White,R;Janoff,A;Gordon,R;Campbell,E
培养的人肺泡巨噬细胞的细胞裂解物含有可检测量的弹性蛋白溶解酶。尽管颗粒弹性蛋白仅在长时间孵育后才溶解，但裂解物很容易水解 t-BOC-丙氨酰-对硝基苯酚-酯。后一种底物的水解被白细胞弹性蛋白酶位点特异性抑制剂 N-ac-(ala) 4-氯甲基酮抑制。此外，先前与 3H 二异丙基氟磷酸酯 (DFP) 一起孵育的浓缩肺泡巨噬细胞裂解物的放射免疫电泳显示，存在与失活的人白细胞弹性蛋白酶共迁移的 DFP 结合材料。人白细胞弹性蛋白酶可在实验动物中引起类似肺气肿的肺部病变；因此，肺泡巨噬细胞清除这种酶可能是从肺部清除这种潜在致病酶的重要途径。
Cell lysates from cultured human alveolar macrophages contain detectable amounts of an elastinolytic enzyme. Although particulate elastin was solubilized only after prolonged incubations, lysates readily hydrolyzed t-BOC-alanyl-p-nitrophenol-ester. Hydrolysis of the latter substrate was inhibited by the leukocyte elastase site-specific inhibitor, N-ac-(ala) 4-chloromethyl ketone. In addition, radioimmunoelectrophoresis of concentrated alveolar macrophage lysates, previously incubated with 3H diisopropyl-phosphofluoridate (DFP), revealed the presence of DFP binding material that comigrated with inactivated human leukocyte elastase. Human leukocyte elastase can cause lung lesions resembling pulmonary emphysema in experimental animals; therefore, the clearance of this enzyme by alveolar macrophages may represent a significant route for the removal of this potentially pathogenic enzyme from the lung.