Two closely related pathways of nicotine catabolism in Arthrobacter nicotinovorans and Nocardioides sp strain JS614
Two closely related pathways of nicotine catabolism in Arthrobacter nicotinovorans and Nocardioides sp strain JS614
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DOI：
10.1007/s00203-007-0340-8
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发表时间：
2008-05-01
影响因子：
2.8
通讯作者：
Brandsch, Roderich
中科院分区：
文献类型：
--
作者：
Ganas, Petra;Sachelaru, Paula;Brandsch, Roderich
已经在A中鉴定了几乎相同的尼古丁分解代谢途径，包括异三聚体钼酶尼古丁和6-羟基-假-氧化尼古丁脱氢酶、6-羟基-L-尼古丁氧化酶、2，6-二羟基-假-氧化尼古丁水解酶和2，6-二羟基吡啶羟化酶。nicotinovorans和Nocardioides sp.JS 614。在两种微生物的提取物中检测到催化相同反应的酶和相似的蛋白抗原。在两种细菌的生长培养基中均检测到烟碱催化剂的两种终产物烟碱蓝和甲胺。A.尼古丁分解代谢基因聚簇在pAO 1上。nicotinovorans，但位于Nocardioides sp. JS 614的染色体上。
A virtually identical nicotine catabolic pathway including the heterotrimeric molybdenum enzyme nicotine and 6-hydroxy-pseudo-oxynicotine dehydrogenase, 6-hydroxy-L-nicotine oxidase, 2,6-dihydroxy-pseudo-oxynicotine hydrolase, and 2,6-dihydroxypyridine hydroxylase have been identified in A. nicotinovorans and Nocardioides sp. JS614. Enzymes catalyzing the same reactions and similar protein antigens were detected in the extracts of the two microorganisms. Nicotine blue and methylamine, two end products of nicotine catabolism were detected in the growth medium of both bacterial species. Nicotine catabolic genes are clustered on pAO1 in A. nicotinovorans, but located chromosomally in Nocardioides sp. JS614.