Spermine is not essential for survival of Arabidopsis
Spermine is not essential for survival of Arabidopsis
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DOI：
10.1016/s0014-5793(03)01395-4
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发表时间：
2004-01-02
期刊：
影响因子：
3.5
通讯作者：
Takahashi, T
中科院分区：
文献类型：
--
作者：
Imai, A;Akiyama, T;Takahashi, T
精胺是多胺生物合成途径的最终产物，广泛存在于大多数生物体中。拟南芥基因组中有两个编码精胺合酶的基因：ACAULIS 5（ACL 5），其功能丧失突变体在茎伸长中表现出严重缺陷，和SPMS。为了阐明精胺在植物中的功能，我们分离了SPMS基因的T-DNA插入突变体。与野生型相比，突变体spms-1的游离精胺和结合精胺水平显著降低，但没有观察到明显的形态表型。我们进一步证实acl 5 -1 spms-1双突变体不含精胺。令人惊讶的是，acl 5 -1 spms-1与野生型完全一样有活力，并且除了由于acl 5 -1而导致的茎生长减少之外没有表现出表型。这些结果表明，精胺不是必需的拟南芥的生存，至少在正常的生长条件下。(C)2003年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Spermine is the final product of the polyamine biosynthetic pathway and is ubiquitously present in most organisms. The genome of Arabidopsis thaliana has two genes encoding spermine synthase: ACAULIS5 (ACL5), whose loss-of-function mutants show a severe defect in stem elongation, and SPMS. In order to elucidate the function of spermine in plants, we isolated a T-DNA insertion mutant of the SPMS gene. Free and conjugated spermine levels in the mutant, designated spms-1, were significantly decreased compared with those in the wildtype, but no obvious morphological phenotype was observed in spms-1 plants. We further confirmed that acl5-1 spms-1 double mutants contained no spermine. Surprisingly, acl5-1 spms-1 was fully as viable as the wild-type and showed no phenotype except for the reduced stem growth due to acl5-1. These results indicate that spermine is not essential for survival of Arabidopsis, at least under normal growth conditions. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.