Development of a Real-Time RT-PCR Assay for the Detection of Crimean-Congo Hemorrhagic Fever Virus
Development of a Real-Time RT-PCR Assay for the Detection of Crimean-Congo Hemorrhagic Fever Virus
复制标题
DOI：
10.1089/vbz.2011.0770
复制
发表时间：
2012-09-01
影响因子：
2.1
通讯作者：
Hewson, Roger
中科院分区：
文献类型：
--
作者：
Atkinson, Barry;Chamberlain, John;Hewson, Roger
克里米亚-刚果出血热 (CCHF) 是一种烈性蜱传疾病，蜱传传播的病死率可达 10-50%，院内传播的病死率高达 80%。欧洲、亚洲和非洲 30 多个国家已报告人间病例。它似乎正在蔓延到新的地区，一些国家在过去 10 年内报告了首例人类 CCHF 病例。我们报告了一种新颖的实时 RT-PCR 检测，旨在放大 CCHF 病毒 S 片段的保守区域。它能够检测所有 7 个 CCHF 组的菌株，包括直到最近代表与人类疾病无关的菌株谱系的 AP92 菌株。该检测的检测限为 5 个目标 RNA 拷贝，并且该检测显示与同一属内的其他病毒或引起类似人类疾病的病毒没有交叉反应。
Crimean-Congo hemorrhagic fever (CCHF) is a virulent tick-borne disease with a case fatality rate ranging from 10-50% for tick-borne transmission, and up to 80% for nosocomial transmission. Human cases have been reported in over 30 countries across Europe, Asia, and Africa. It appears to be spreading to new areas with several countries reporting their first human cases of CCHF disease within the past 10 years. We report a novel real-time RT-PCR assay designed to amplify a conserved region of the CCHF virus S segment. It is capable of detecting strains from all 7 groups of CCHF, including the AP92 strain that until recently represented a lineage of strains that were not associated with human disease. The limit of detection of the assay is 5 copies of target RNA, and the assay shows no cross-reactivity with other viruses from within the same genus, or with viruses causing similar human disease.