RhoBTB2 (DBC2) functions as tumor suppressor via inhibiting proliferation, preventing colony formation and inducing apoptosis in breast cancer cells
RhoBTB2 (DBC2) functions as tumor suppressor via inhibiting proliferation, preventing colony formation and inducing apoptosis in breast cancer cells
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DOI：
10.1016/j.gene.2011.07.018
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发表时间：
2011-10-15
期刊：
影响因子：
3.5
通讯作者：
Qu, Xun
中科院分区：
文献类型：
--
作者：
Mao, Haiting;Zhang, Lining;Qu, Xun
RhoBTB 2是最近从散发性乳腺癌中分离出来的一个抑癌基因。虽然RhoBTB 2在乳腺癌和多种癌症中经常丢失，但其抗肿瘤作用仍不清楚。本研究构建了重组表达载体pEGFP-N1-RhoBTB 2，并将其转染RhoBTB 2阴性乳腺癌细胞系T-47 D。通过荧光显微镜、RT-PCR和Western blot鉴定稳定的转化细胞。通过MIT测定法测量细胞活力。集落形成实验检测乳腺癌细胞集落形成率。苏木精-伊红染色观察凋亡细胞的形态学变化。流式细胞仪检测细胞凋亡率。transwell法检测细胞侵袭和迁移能力。RhoBTB 2在乳腺癌细胞中的过表达显著抑制肿瘤细胞的增殖和集落形成。此外，RhoBTB 2还能提高RhoBTB 2过表达的乳腺癌细胞的凋亡率，并引起典型的凋亡形态学改变。RhoBTB 2对乳腺癌细胞的侵袭和迁移能力无明显影响。因此。RhoBTB 2是一个与乳腺癌相关的重要抑癌基因，可能通过抑制肿瘤细胞增殖、阻止集落形成、促进肿瘤细胞凋亡等途径发挥抗肿瘤作用。然而，RhoBTB 2抗肿瘤作用背后的确切机制需要进一步研究。(C)2011 Elsevier B. V.保留所有权利。
RhoBTB2 was isolated recently as a tumor suppressor gene from sporadic breast cancer. Although RhoBTB2 was found to be frequently lost in breast cancer and a variety of cancers, its antitumor effect, however, remains unclear. In this study, we constructed a recombinant expression vector pEGFP-N1-RhoBTB2 and transfected it into RhoBTB2-negative breast tumor cell line T-47D. Stable transformanted cells were identified by fluorescence microscope, RT-PCR and Western blot. Cell viability was measured by MIT assay. Colony forming efficiency of breast tumor cells was detected by colony formation assay. Morphological change of apoptotic cells was observed by hematoxylin-eosin staining. Apoptotic ratio was determined by flow cytometry. Cell invasion and migration ability assay were performed using transwell system. Overexpression of RhoBTB2 in breast tumor cells significantly inhibited the proliferation and colony formation of tumor cells. In addition, RhoBTB2 also elevated the apoptotic ratio and caused typical changes of apoptotic morphology in breast tumor cells of RhoBTB2 overexpression. But RhoBTB2 did not influence the invasion and migration ability of breast tumor cells. Therefore. RhoBTB2 is an important tumor suppressor gene related with breast cancer and may play antitumor roles by inhibiting proliferation, preventing colony formation and promoting the apoptosis of tumor cells. However, the precise mechanism behind the antitumor effects of RhoBTB2 needs to be investigated further. (C) 2011 Elsevier B.V. All rights reserved.