Purification and Characterization of the Corticosteroid 1lβ-Dehydrogenase Component of the Rat Liver 1lβ-Hydroxysteroid Dehydrogenase Complex*
Purification and Characterization of the Corticosteroid 1lβ-Dehydrogenase Component of the Rat Liver 1lβ-Hydroxysteroid Dehydrogenase Complex*
复制标题
大鼠肝脏 1lβ-羟基类固醇脱氢酶复合物中皮质类固醇 1lβ-脱氢酶成分的纯化和表征*
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
C. Monder
中科院分区：
文献类型：
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作者：
V. Lakshmi;C. Monder
我们提出11β-羟基类固醇脱氢酶是由具有11β-脱氢酶和11-还原酶活性的结构独立单元组成的。我们现在报道纯化大鼠肝脏11β-脱氢酶到明显的同质性。从微粒体开始，琼脂糖- nadp亲和层析实现了800倍的纯化。11-还原酶伴随纯化。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、氨基酸端基分析和免疫沉淀法测定11β-脱氢酶的均匀性。末端氨基酸为蛋氨酸。单体mol wt是34000。这种酶被发现是一种糖蛋白。从氨基端鉴定出40个氨基酸单位的序列。氨基末端区域是非极性的。与未纯化的110-脱氢酶不同，均质酶呈现直线曲线。皮质酮的Michaelis常数为1.83±0.06 μM，皮质醇为17.3±2.24 μM . ...
We have proposed that 11β-hydroxysteroid dehydrogenase is composed of structurally independent units with 11β-dehydrogenase and 11-reductase activities. We now report the purification of rat liver 11β-dehydrogenase to apparent homogeneity. Starting with microsomes, 800-fold purification was achieved with agarose-NADP affinity chromatography. No 11- reductase accompanied the purification. Homogeneity of 11β- dehydrogenase was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and amino acid end-group analysis and immunoprecipitation. The terminal amino acid was methionine. Monomer mol wt was 34,000. The enzyme was found to be a glycoprotein. A sequence of 40 amino acid units was identified from the amino end. The amino-terminal region was found to be highly nonpolar. Unlike unpurified microsomal 110- dehydrogenase, which showed curvilinear Eadie plots, homogeneous enzyme gave rectilinear plots. Michaelis constants were 1.83 ± 0.06 μM for corticosterone and 17.3 ± 2.24 μM for cortisol. ...
明显盐皮质激素过多综合征：与 11 β-脱氢酶和 5 β-还原酶缺乏相关以及皮质类固醇代谢的一些后果。
DOI：
10.1210/jcem-63-3-550
发表时间：
1986
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
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作者：
Monder,C;Shackleton,CH;Bradlow,HL;New,MI;Stoner,E;Iohan,F;Lakshmi,V
通讯作者：
Lakshmi,V