MODERATE RUNNING EXERCISE AUGMENTS GLYCOSAMINOGLYCANS AND THICKNESS OF ARTICULAR-CARTILAGE IN THE KNEE-JOINT OF YOUNG BEAGLE DOGS
MODERATE RUNNING EXERCISE AUGMENTS GLYCOSAMINOGLYCANS AND THICKNESS OF ARTICULAR-CARTILAGE IN THE KNEE-JOINT OF YOUNG BEAGLE DOGS
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DOI：
10.1002/jor.1100060205
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发表时间：
1988-03-01
影响因子：
2.8
通讯作者：
HELMINEN, HJ
中科院分区：
文献类型：
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作者：
KIVIRANTA, I;TAMMI, M;HELMINEN, HJ
采用番红O和过碘酸希夫（PAS）染色组织切片显微分光光度法，测定了运动对犬关节软骨厚度和糖胺聚糖（GAG）含量的局部影响。将雌性比格犬圈养在单独的笼中（底部0.9 × 10）。1.2 m），分为跑步组（n = 6）和对照组（n = 8）。训练计划从15周开始。在随后的10周内，狗习惯于在倾斜15 °的跑步机上跑步。上坡此后，狗每天跑1小时，每周5天，以4公里/小时的速度跑了15周。40周龄时，将犬处死，从右膝（后膝）关节的11个不同解剖位置采集组织学样本。股骨外髁和髌骨表面的未钙化软骨厚度增加19-23%，而训练软骨的其他部分的增加较小。钙化的软骨没有显示厚度改变。股骨髁顶点的总GAG增加了28%，内侧多于外侧。增加的GAG似乎主要是硫酸软骨素，并局限于中间，深层，甚至在钙化区，而浅表区没有显示出变化。在中间区和深部区，但在钙化软骨中，非GAG寡糖伴随增加。从增强的GAG含量和厚度判断，认为适度的跑步运动局部改变了承受最高负荷盈余的区域的年轻关节软骨的生物学特性。
The local influences of physical exercise on thickness and glycosaminoglycan (GAG) content of canine articular cartilage were measured by microspectrophotometry of Safranin O- and periodic acid-Schiff (PAS)-stained tissue sections. Female Beagle dogs were housed in individual cages (bottom 0.9 .times. 1.2 m) and divided into runner (n = 6) and control (n = 8) groups. The training program started at the age of 15 weeks. During the subsequent 10 weeks, the dogs were accustomed to running on a treadmill inclined 15.degree. uphill. Thereafter, the dogs ran 1 h daily, 5 days a week, at a speed of 4 km/h for 15 weeks. At the age of 40 weeks, the dogs were killed, and the samples for histology were taken from 11 different anatomical locations of the right knee (stifle) joint. The thickness of the uncalcified cartilage increased 19-23% on the lateral condyle and patellar surface of the femur, whereas the enhancement was smaller in other parts of the trained cartilage. The calcified cartilage did not show thickness alterations. Total GAGs were augmented by 28% in the summits on the femoral condyles, more on the medial than lateral side. The increased GAGs appeared to be predominantly chondroitin sulphates and were localized in the intermediate, deep, and even in the calcified zones, whereas the superficial zone did not show changes. There was a concomitant increase of non-GAG oligosaccharides in the intermediate and deep zones, but not in the calcified cartilage. As judged from the enhanced GAG content and thickness, it is considered that moderate running exercise locally alters the biological properties of young articular cartilage at regions bearing the highest loading surplus.