A new neutrophil candida killing test: chromium-51 release from Candida guilliermondii.
A new neutrophil candida killing test: chromium-51 release from Candida guilliermondii.
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一种新的中性粒细胞念珠菌杀灭试验：吉里蒙念珠菌释放 51 铬。
DOI：
10.1136/jcp.33.8.757
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发表时间：
1980
影响因子：
3.4
通讯作者：
J. Child
中科院分区：
文献类型：
--
作者：
S. Martin;A. Ghoneim;J. Child
使用季也蒙念珠菌作为试验微生物，通过铬-51（51 Cr）释放技术测定杀中性粒细胞念珠菌功能。不同的中性粒细胞的影响：念珠菌的比例，51铬释放的时间过程中，不同的血清浓度的影响，和该技术的再现性进行了研究。来自33个实验的结果，使用来自35个正常供体和6个已知中性粒细胞缺陷的患者的中性粒细胞，表明该技术是一种可靠的和可重复的念珠菌杀灭测定。铬释放测定法具有易于执行且不易于主观误差的优点。
Neutrophil candidacidal function was measured by a chromium-51 (51Cr) release technique, using Candida guilliermondii as the test organism. The effect of varying the neutrophil:candida ratio, the time course of 51Cr release, the effect of different serum concentrations, and the reproducibility of the technique were investigated. Results from 33 experiments, using neutrophils from 35 normal donors and six patients with known neutrophil defects, show the technique to be a reliable and reproducible assay of candida killing. The chromium release assay has the advantage of being easy to perform and not liable to subjective errors.