WILD-TYPE OVULE DEVELOPMENT IN ARABIDOPSIS-THALIANA - A LIGHT-MICROSCOPE STUDY OF CLEARED WHOLE-MOUNT TISSUE
WILD-TYPE OVULE DEVELOPMENT IN ARABIDOPSIS-THALIANA - A LIGHT-MICROSCOPE STUDY OF CLEARED WHOLE-MOUNT TISSUE
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DOI：
10.1046/j.1365-313x.1995.07050731.x
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发表时间：
1995-05-01
期刊：
影响因子：
7.2
通讯作者：
PRUITT, RE
中科院分区：
文献类型：
--
作者：
SCHNEITZ, K;HULSKAMP, M;PRUITT, RE
详细描述了拟南芥野生型胚珠发育的形态。描述了从胚珠突起形成到八核胚乳形成的全过程。这项研究是基于染色，随后光学透明的整装胚珠的光显微镜分析。它是由碘化丙啶染色的整装胚珠的共聚焦激光扫描显微镜的补充。已经表明，这两种技术的组合在很大程度上消除了对切片的需要，因此允许在拟南芥中的大量胚珠的快速形态检查。胚珠是一个相对简单的器官。在发育过程中，三个离散的主要模式元素是沿着近端-远端轴：珠心在远端（窝藏大孢子/配子体谱系），合点（两侧被珠被）和索（包括维管股）在近端。这三个图案元素在非常早期的阶段就已经出现了，当最初形成的由一排排均匀的细胞组成的突起转化为图案化的原基时。随后的形态发生导致每个图案元素的形态特征的表现。这是可能的解剖这一发展过程中不同的，形态上可辨别的步骤，在高分辨率。提出了一个胚珠发育阶段的分类方案，这是基于胚珠特异性，离散，易于评分的标记。
A detailed morphological description of wild-type ovule development in Arabidopsis thaliana is presented. The entire process from the formation of the ovule protrusion until the eight-nuclear endosperm stage is described. The study is based on a light-microscopical analysis of stained and subsequently optically cleared whole-mount ovules. It is supplemented by confocal laser scanning microscopy of propidium iodide-stained whole-mount ovules. It has been shown that the combination of both techniques eliminates the need for sections to a large extent, and hence allows the rapid morphological inspection of a large number of ovules in Arabidopsis. The ovule constitutes a relatively simple organ. During development, three discrete major pattern elements are laid down along the proximal-distal axis: the nucellus at the distal end (harbors the megaspore/gametophyte lineage), the chalaza (flanked by the integuments) and the funiculus (includes the vascular strand) at the proximal end. These three pattern elements already appear at a very early stage, when the initially formed protrusion, consisting of files of uniform cells, is transformed into a patterned primordium. Subsequent morphogenesis results in the manifestation of the morphological characters of each pattern element. It was possible to dissect this developmental process into distinct, morphologically discernible steps at a high resolution. A classification scheme of ovule developmental stages is proposed, which is based on ovule-specific, discrete, and easy-to-score markers.