Hypomethylation of the 14-3-3σ promoter leads to increased expression in non-small cell lung cancer.
Hypomethylation of the 14-3-3σ promoter leads to increased expression in non-small cell lung cancer.
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DOI：
10.1002/gcc.20904
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发表时间：
2011-10
影响因子：
3.7
通讯作者：
Martinez, Jesse D.
中科院分区：
文献类型：
--
作者：
Radhakrishnan, Vijayababu M.;Jensen, Taylor J.;Cui, Haiyan;Futscher, Bernard W.;Martinez, Jesse D.
The 14-3-3 proteins are a set of seven highly conserved proteins that have recently been implicated in having a role in human tumorigenesis. However, the mechanism by which 14-3-3 proteins may act in this capacity is not well understood. In this study, we examined the expression of one of the 14-3-3 family members, 14-3-3σ since it was shown previously to be aberrantly altered in human tumors. Using quantitative rtPCR and immunohistochemistry, we found that the expression levels of 14-3-3σ were elevated in the majority of human non-small cell lung cancers (NSCLC) we examined. Surprisingly, we found that the 14-3-3σ gene was hypomethylated in lung tumors relative to normal lung tissue suggesting that decreased DNA methylation resulted in increased expression of 14-3-3σ in NSCLC. We also determined the gene copy number for 14-3-3σ in tumor samples and found no significant correlation with elevated mRNA expression. And also no mutations were found in 14-3-3σ gene. Overall, our data suggests that misregulated expression of 14-3-3σ gene may be due to altered methylation status.
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