Curcumin exerts a protective effect against premature ovarian failure in mice.
Curcumin exerts a protective effect against premature ovarian failure in mice.
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DOI：
10.1530/jme-17-0214
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发表时间：
2018-04
影响因子：
3.5
通讯作者：
Cui Y
中科院分区：
文献类型：
--
作者：
Yan Z;Dai Y;Fu H;Zheng Y;Bao D;Yin Y;Chen Q;Nie X;Hao Q;Hou D;Cui Y
本研究旨在探讨姜黄素对d-半乳糖（d-gal）诱导的小鼠卵巢早衰（POF）的保护作用。每日皮下注射d-半乳糖（200 mg/kg/d），连续42 d建立小鼠POF模型。姜黄素组小鼠在d-gal治疗的同时腹腔注射姜黄素（100 mg/kg/天），连续42天。观察卵巢功能、氧化应激和细胞凋亡。姜黄素组P、E2、SOD水平显著高于d-gal组，FSH、LH、MDA水平显著低于d-gal组。姜黄素组的原始卵泡比例也明显高于d-gal组。此外，与d-gal组相比，姜黄素处理后，颗粒细胞中Sod2、Cat、8-OhdG、4-HNE、NTY和衰老相关蛋白P16的表达水平显著降低，Amh表达水平升高，细胞凋亡减少。姜黄素组p-Akt、Nrf2和HO-1蛋白表达水平显著高于d-gal组，凋亡相关的cleaved - caspase-3和-9蛋白表达水平显著低于d-gal组。综上所述，姜黄素通过Nrf2/HO-1和PI3K/Akt信号通路有效抑制d-gal诱导的氧化应激、细胞凋亡和卵巢损伤，提示姜黄素是一种潜在的POF保护剂。
This study was designed to investigate the protective effect of curcumin against d-galactose (d-gal)-induced premature ovarian failure (POF) in mice. A mouse POF model was induced by subcutaneous injection of d-gal (200 mg/kg/day) daily for 42 days. Mice in the curcumin group received both d-gal treatment and intraperitoneal injection of curcumin (100 mg/kg/day) for 42 days. Ovarian function, oxidative stress and apoptosis were evaluated. The P, E2 and SOD levels were higher, and the FSH, LH and MDA levels were significantly lower in the curcumin group than those in the d-gal group. The proportion of primordial follicles was also significantly higher in the curcumin group than that in the d-gal group. In addition, curcumin treatment after d-gal administration resulted in significantly lower Sod2, Cat, 8-OhdG, 4-HNE, NTY and senescence-associated protein P16 expression levels, higher Amh expression levels and less apoptosis in granulosa cells than was observed in the d-gal group. Moreover, the p-Akt, Nrf2 and HO-1 protein expression levels were significantly higher and the apoptosis-related cleaved caspase-3 and -9 protein expression levels were markedly lower in the curcumin group than in the d-gal group. In conclusion, curcumin effectively inhibited d-gal-induced oxidative stress, apoptosis and ovarian injury via a mechanism involving the Nrf2/HO-1 and PI3K/Akt signaling pathways, suggesting that curcumin is a potential protective agent against POF.