Cell Adhesion, Morphology, and Metabolism Variation via Acoustic Exposure within Microfluidic Cell Handling Systems
Cell Adhesion, Morphology, and Metabolism Variation via Acoustic Exposure within Microfluidic Cell Handling Systems
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DOI：
10.1002/advs.201902326
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发表时间：
2019-12-18
期刊：
影响因子：
15.1
通讯作者：
Neild, Adrian
中科院分区：
文献类型：
--
作者：
Devendran, Citsabehsan;Carthew, James;Neild, Adrian
声场能够以多种方式操纵微流控芯片的封闭且高度受控的环境中包含的生物样本。使用声流改变流体流动和辐射力来控制细胞位置具有重要的临床和生命科学应用。虽然这些声学机制的基本实现已经取得了重大进展，但对细胞的相关生物效应还相当缺乏了解。通常使用单一、简单的活力测定来证明高比例的活细胞。然而，这项研究的结果表明，声暴露可以抑制细胞附着，减少细胞扩散，最有趣的是增加细胞代谢活动，所有这些都不会对生存率产生任何影响。这具有重要意义，表明仅死亡率研究不足以评估生物相容性，但进一步表明细胞的物理操作也可用于影响其生物活性。
Acoustic fields are capable of manipulating biological samples contained within the enclosed and highly controlled environment of a microfluidic chip in a versatile manner. The use of acoustic streaming to alter fluid flows and radiation forces to control cell locations has important clinical and life science applications. While there have been significant advances in the fundamental implementation of these acoustic mechanisms, there is a considerable lack of understanding of the associated biological effects on cells. Typically a single, simple viability assay is used to demonstrate a high proportion of living cells. However, the findings of this study demonstrate that acoustic exposure can inhibit cell attachment, decrease cell spreading, and most intriguingly increase cellular metabolic activity, all without any impact upon viability rates. This has important implications by showing that mortality studies alone are inadequate for the assessment of biocompatibility, but further demonstrates that physical manipulation of cells can also be used to influence their biological activity.