Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis
Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis
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DOI：
10.1099/00207713-50-3-1351
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发表时间：
2000-05-01
影响因子：
2.8
通讯作者：
Statzell-Tallman, A
中科院分区：
文献类型：
--
作者：
Fell, JW;Boekhout, T;Statzell-Tallman, A
利用大亚基rDNA的D1/D2区序列分析，确定了337株担子菌酵母和酵母样真菌的分子系统，分别属于18个变形属和24个远形属230种。这些数据与其他担子菌属真菌的已发表序列进行了比较。结果表明，酵母菌种属分布于微菌门、孢子菌门、木耳门和红担子门；膜菌属的银耳门、毛孢门、丝状菌门和囊状菌门；以及Ustilaginales、Microstromatales和Malasseziales的ustilaginomyates分支。Bensingtonia、隐球菌（Cryptococcus）、红球菌（Rhodotorula）和孢子菌（Sporobolomyces）等属是多系的，即它们出现在两个或多个分支中。相反，其他属，如球孢菌属、囊泡菌属、霉属。Filobasidiella, Filobasidium, Kondoa, Kurtzmanomyces, Leucosporidium, Rhodosporidium， Sporidiobolus和Udeniomyces是单系的。大多数物种可以通过D1/D2分析来识别，尽管需要内部转录间隔区来区分近亲物种。基因间间隔区被推荐用于物种和菌株的进一步分化。
The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA. The data were compared with published sequences of other basidiomycetous fungi. The results demonstrated that the yeast species and genera are phylogenetically distributed among the Microbotryum, Sporidiobolus, Agaricostilbum and Erythrobasidium clades of the Urediniomycetes; the Tremellales, Trichosporonales ord. nov., Filobasidiales and Cystofilobasidiales clades of the Hymenomycetes; and the Ustilaginales, Microstromatales and Malasseziales clades of the Ustilaginomycetes. Genera such as Bensingtonia, Cryptococcus, Rhodotorula and Sporobolomyces are polyphyletic, i.e. they occur in two or more clades. In contrast, other genera, e.g. Bullera, Cystofilobasidium, Fellomyces. Filobasidiella, Filobasidium, Kondoa, Kurtzmanomyces, Leucosporidium, Rhodosporidium, Sporidiobolus and Udeniomyces, are monophyletic. The majority of the species can be identified using D1/D2 analyses, although the internal transcribed spacer region is required to distinguish closely related species. The intergenic spacer region is recommended for additional differentiation of species and strains.