Metabolomes of bumble bees reared in common garden conditions suggest constitutive differences in energy and toxin metabolism across populations
Metabolomes of bumble bees reared in common garden conditions suggest constitutive differences in energy and toxin metabolism across populations
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DOI：
10.1016/j.jinsphys.2023.104581
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发表时间：
2023-11-02
影响因子：
2.2
通讯作者：
Dillon，Michael E.
中科院分区：
文献类型：
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作者：
Keaveny，Ellen C.;Helling，Mitchell R.;Dillon，Michael E.
变温动物的耐冷能力在物种和种群之间存在显着差异。耐冷性的变化可以反映基因组和转录组的差异，这些差异赋予细胞水平的寒冷保护；此外，蛋白质功能和丰度的变化可能会被其他细胞成分改变，因为暴露在寒冷中的昆虫的代谢组通常会发生变化。即使没有寒冷的挑战，来自不同种群的昆虫的细胞组成也可能有所不同，这可能会改变耐冷性，但对野生种群代谢组的组成性差异的研究仍然很少。为了解决这一差距，我们在常见花园条件下饲养了从俄勒冈州和加利福尼亚州（美国）收集的具有不同耐冷性（CTmin分别为-6°C和0°C）的熊蜂，并使用非靶向LC-MS/MS测量了后代代谢组。俄勒冈州蜜蜂与碳水化合物（山梨醇、乳糖醇、麦芽糖醇和山梨醇 6-磷酸）和氨基酸（羟脯氨酸、鸟氨酸和组胺）代谢相关的代谢物水平较高。俄勒冈州蜜蜂和加利福尼亚州蜜蜂之间的外源代谢物（可能来自饮食）也有所不同，这表明毒素代谢存在种群水平差异。总体而言，我们的结果揭示了在普通花园条件下饲养的大黄蜂与在不同地点收集的蜂王的代谢组的构成差异，尽管以前没有接触过寒冷。
Cold tolerance of ectotherms can vary strikingly among species and populations. Variation in cold tolerance can reflect differences in genomes and transcriptomes that confer cellular-level protection from cold; additionally, shifts in protein function and abundance can be altered by other cellular constituents as cold-exposed insects often have shifts in their metabolomes. Even without a cold challenge, insects from different populations may vary in cellular composition that could alter cold tolerance, but investigations of constitutive differences in metabolomes across wild populations remain rare. To address this gap, we rearedBombus vosnesenskiiqueens collected from Oregon and California (USA) that differ in cold tolerance (CTmin= -6 °C and 0 °C, respectively) in common garden conditions, and measured offspring metabolomes using untargeted LC-MS/MS. Oregon bees had higher levels of metabolites associated with carbohydrate (sorbitol, lactitol, maltitol, and sorbitol-6-phosphate) and amino acid (hydroxyproline, ornithine, and histamine) metabolism. Exogenous metabolites, likely derived from the diet, also varied between Oregon and California bees, suggesting population-level differences in toxin metabolism. Overall, our results reveal constitutive differences in metabolomes for bumble bees reared in common garden conditions from queens collected in different locations despite no previous cold exposure.