:H7 transcriptome datasets for comparison of RNA-seq and microarray platforms
:H7 transcriptome datasets for comparison of RNA-seq and microarray platforms
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：用于比较 RNA-seq 和微阵列平台的 H7 转录组数据集
DOI：
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
N. Holden
中科院分区：
文献类型：
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作者：
Ewa Grabowiecka;David Martin;L. Crozier;N. Holden
研究差异基因表达和调控适应性的全转录组分析可以在两种不同的技术平台上进行：通过探针杂交到微阵列或通过RNAseq进行深度测序。由于它们在基因组覆盖率、敏感性和成本方面存在差异，因此需要进行可靠的比较以确定选择的平台。在这里，我们展示了从RNA-seq和微阵列平台上获得的对菠菜产生细胞毒性的大肠杆菌（VTEC）的整个转录反应的数据集，并对数据集进行了比较（可在Array Express上获得：E-MTAB-3249， E-MTAB-4120, E-MTAB-7441）。爱思唯尔公司版权所有，2018年出版。这是一篇基于CC BY许可（http://creativecommons.org/licenses/by/4.0/）的开放获取文章。
Whole transcriptome analysis to investigate differential gene expression and regulatory adaption can be carried out on two different technological platforms: by probe hybridisation to microarrays or by RNAseq for deep sequencing. Since there are difference in terms of their genome coverage, sensitivity and cost, there is a requirement for robust comparisons to determine the platform of choice. Here, we present datasets for the whole transcriptional response verocytoxi-genic Escherichia coli (VTEC) obtained from RNA-seq and microarray platforms in response to spinach, together with a comparison between the datasets (available at Array Express: E-MTAB-3249, E-MTAB-4120, E-MTAB-7441). Crown Copyright & 2018 Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).