Measurement of estrogen surge during pseudopregnancy in rats by radioimmunoassay.
Measurement of estrogen surge during pseudopregnancy in rats by radioimmunoassay.
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放射免疫法测定大鼠假孕期间雌激素激增。
DOI：
10.1095/biolreprod1.4.378
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发表时间：
1969
影响因子：
3.6
通讯作者：
G. Abraham
中科院分区：
文献类型：
--
作者：
A. Shaikh;G. Abraham
用放射免疫法测定假孕不同时期大鼠卵巢静脉血浆中雌二醇的含量。假孕第2天和第3天的雌二醇水平均低于50个/毫升血浆。在第4天，10：00-12：00小时的平均峰值为682PCGS-‘ml。然后，这些价值逐渐下降。在14：00-16：00小时，他们是610钉/英里。18：00-20：00时为467针/英里，22：00-24：00时为225针/英里。在第5天的10：00-12：00小时，该值为297pegs/mi。而第4天22：00-24：00与第5天10：00-12：00之间无显著差异。假孕第4天不同时间测定值均不呈线性回归，平均值差异较大。因此，在第4天，卵巢维持雌激素分泌平台期一段时间。这一雌激素分泌平台期可能在子宫对蜕膜细胞反应或着床的敏感化过程中起重要作用。总而言之，研究人员发现，雌激素高峰出现在假孕第4天上午10点之前，而不是像其他使用雌激素生物学标准的工作者所报告的那样，发生在下午晚些时候。
Estradiol was measured in rat ovarian venous blood plasma at different stages of pseudopregnancy by radioimmunoassay. Estradiol levels on Days 2 and 3 of pseudopregnancy were less than 50 pegs/mI plasma. On Day 4 the assay showed a mean peak value of 682 pcgs-’ml at 10:00-12:00 hr. The values then gradually declined. They were 610 pegs/mI at 14:00-16:00 hr. 467 pegs/mI at 18:00-20:00 hr, and 225 pegs/mI at 22:00-24:00 hr. At 10:00-12:00 hr on Day 5, the values were 297 pegs/mI. There was, however, no significant difference between the values at 22:00-24:00 hr on Day 4 and 10:00-12:00 hr on Day 5. The values determined at various times on Day 4 of pseudopregnancv did not show a linear regression and there was a great variation about the mean. It is therefore suggested that the ovary maintains a plateau of estrogen secretion for some hours on Day 4. This plateau of estrogen secretion probably is of importance in uterine sensitization for decidual cell reaction or implantation. In conclusion, the estrogen surge was found to occur not later than 10:00 hr on Day 4 of pseudopregnancy, rather than during the latter part of the afternoon as has been reported by other workers using biological criteria for estrogen