Ultraviolet C light for Acinetobacter baumannii wound infections in mice: potential use for battlefield wound decontamination?
Ultraviolet C light for Acinetobacter baumannii wound infections in mice: potential use for battlefield wound decontamination?
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紫外线 C 光治疗小鼠鲍曼不动杆菌伤口感染：战场伤口净化的潜在用途？
DOI：
10.1097/ta.0b013e31825c149c
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发表时间：
2012-09
期刊：
影响因子：
--
通讯作者：
Hamblin MR
中科院分区：
文献类型：
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作者：
Dai T;Murray CK;Vrahas MS;Baer DG;Tegos GP;Hamblin MR
自中东冲突开始以来，美国陆军医生注意到，在伊拉克受伤并最初接受治疗的美国士兵中，多药耐药鲍曼不动杆菌感染率很高。在这项研究中，我们利用小鼠模型研究了紫外线C （UVC）光预防多重耐药鲍曼不动杆菌伤口感染的作用。研究人员从一名被派往伊拉克的受伤美军士兵身上分离出一种具有多重耐药性的鲍曼不动杆菌，感染了局部皮肤擦伤和全层烧伤的小鼠。将质粒pmf385中的luxCDABE操纵子克隆到鲍曼不动杆菌中。这使得使用生物发光成像对小鼠感染程度进行实时监测成为可能。UVC光于接种鲍曼不动杆菌30分钟后照射小鼠伤口。几组未暴露在紫外线下的伤口感染小鼠作为对照。体外研究表明，鲍曼不动杆菌细胞在紫外线照射下的失活程度远低于对哺乳动物细胞产生类似效果所需的紫外线照射量。在动物研究中观察到，UVC（擦伤3.24 J/cm2，烧伤2.59 J/cm2）与未处理的对照组相比，UVC处理伤口的细菌负荷显著减少了约10倍（擦伤p = 0.004，烧伤p = 0.019）。在3.24 J/cm2的UVC照射下，用免疫荧光法观察小鼠皮肤损伤后的DNA损伤；然而，病变在72小时内得到了广泛修复。这些结果表明，UVC可能有助于预防与战斗相关的伤口感染。
Since the beginning of the conflicts in the Middle East, US Army physicians have noted a high rate of multidrug-resistant Acinetobacter baumannii infections among US soldiers wounded and initially treated in Iraq. In this study, we investigated the use of ultraviolet C (UVC) light for prevention of multidrug-resistant A. baumannii wound infections using mouse models. Partial-thickness skin abrasions and full-thickness burns in mice were infected with a multidrug-resistant A. baumannii isolate recovered from a wounded US soldier deployed to Iraq. The luxCDABE operon, which was contained in plasmid pMF 385, was cloned into the A. baumannii strain. This allowed real-time monitoring of the extent of infection in mice using bioluminescence imaging. UVC light was delivered to the mouse wounds at 30 minutes after the inoculation of A. baumannii. Groups of infected mouse wounds without being exposed to UVC served as the controls. In vitro studies demonstrated that A. baumannii cells were inactivated at UVC exposures much lower than those needed for a similar effect on mammalian cells. It was observed in animal studies that UVC (3.24 J/cm2 for abrasions and 2.59 J/cm2 for burns) significantly reduced the bacterial burdens in UVC-treated wounds by approximately 10-fold compared with nontreated controls (p = 0.004 for abrasions, p = 0.019 for burns). DNA lesions were observed by immunofluorescence in mouse skin abrasions immediately after a UVC exposure of 3.24 J/cm2; however, the lesions were extensively repaired within 72 hours. These results suggested that UVC may be useful in preventing combat-related wound infections.