Ocular tissue imaging using ultrahigh-resolution, full-field optical coherence tomography
Ocular tissue imaging using ultrahigh-resolution, full-field optical coherence tomography
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DOI：
10.1167/iovs.04-0584
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发表时间：
2004-11-01
影响因子：
4.4
通讯作者：
Le Gargasson, JF
中科院分区：
文献类型：
--
作者：
Grieve, K;Paques, M;Le Gargasson, JF
目的.超高分辨率、全场光学相干断层扫描（OCT）使用白色光源，无需扫描即可实现二维、无创断层成像。本研究的目的是将全视野OCT应用于眼组织成像，以探索该技术的能力。该全视场OCT系统使用具有钨卤素光源的Linnik型干涉仪。空间分辨率为0.9 × 0.7 μ m（横向X轴向）。在浸没条件下检查未染色的组织样品（角膜、透镜、视网膜、脉络膜和巩膜）以及大鼠、小鼠和猪的整只未固定眼睛。电荷耦合器件（CCD）照相机记录一对干涉图像，该干涉图像被组合以正面显示（即，在x-y平面中）真实的时间的断层摄影图像。包括10个原始图像的总和的每个断层图像的采集时间为1秒的量级。后处理允许通过图像堆栈进行体积导航以及三维（3D）成像。在分离的组织样品中实现了细胞水平的分辨率。正面（x-y）图像显示角膜上皮和基质细胞、透镜纤维、神经纤维、主要血管和视网膜色素上皮细胞。在x-z重建中，角膜和视网膜以及小动脉和小静脉内的细胞层被清楚地定义。在白化病动物中实现了经巩膜视网膜成像。超高分辨率、全视野OCT允许对具有高穿透深度的未染色眼组织进行细胞级成像。虽然目前的系统是不适合临床使用，这种简单的技术具有潜在的体内眼部检查，目前正在开发一种新的系统。
PURPOSE. Ultrahigh-resolution, full-field optical coherence tomography (OCT), which uses a white light source, allows bidimensional, noninvasive tomographic imaging without scanning. The goal of the present study was to apply full-field OCT to ocular tissue imaging in an attempt to explore the capabilities of the technique.METHODS. This full-field OCT system uses a Linnik-type interferometer with a tungsten-halogen source. The spatial resolution is 0.9 X 0.7 mum (transverse X axial). Unstained tissue samples (cornea, lens, retina, choroid, and sclera) and whole, unfixed eyes of rat, mouse, and pig were examined under immersion. A charge-coupled device (CCD) camera recorded a pair of interferometric images that were combined to display en face (i.e., in the x-y plane) tomographic images in real time. The acquisition time per tomographic image, which includes summation of 10 raw images, was on the order of 1 s. Post-processing allows volumetric navigation through the image stack as well as three-dimensional (3D) imaging.RESULTs. Cellular-level resolution was achieved in isolated tissue samples. En face (x-y) images revealed corneal epithelial and stromal cells, lens fibers, nerve fibers, major vessels, and retinal pigment epithelial cells. In x-z reconstructions, cellular layers within the cornea and retina and arterioles and venules were clearly defined. Transscleral retinal imaging was achieved in albino animals.CONCLUSIONS. Ultrahigh-resolution, full-field OCT allows cellular-level imaging of unstained ocular tissues with high penetration depth. Although the current system is unsuitable for clinical use, this simple technique has potential for in vivo ocular examination, for which a new system is currently under development.