DETERMINATION OF SUBCUTANEOUS TUMOR SIZE IN ATHYMIC (NUDE) MICE
DETERMINATION OF SUBCUTANEOUS TUMOR SIZE IN ATHYMIC (NUDE) MICE
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DOI：
10.1007/bf00300234
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发表时间：
1989-01-01
影响因子：
3
通讯作者：
REYNOLDS, CP
中科院分区：
文献类型：
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作者：
TOMAYKO, MM;REYNOLDS, CP
无胸腺（裸）小鼠是用于研究体内人类肿瘤的生物学和对治疗的反应的有用模型。最近的文献调查显示，使用19种不同的公式来确定裸鼠异种移植皮下肿瘤的大小（2种用于确定肿瘤面积，3种用于肿瘤直径，14种用于计算肿瘤体积）。我们比较了19个公式预测的体积、面积和直径，并将其与在裸鼠中建立的50个肿瘤的实际重量（从0.46 g到22.0 g）进行了比较，这些肿瘤作为人细胞系的异种移植物。除了确定每个公式预测相对肿瘤大小的程度外，我们还分析了每个公式估计实际肿瘤质量的程度。椭球体体积公式（π/ 6倍。L. times. W. Times. H和1/2 ×L. times. W. Times. H）最适合估计肿瘤质量（r = 0.93），而直径测量与肿瘤质量的相关性较差（r < 0.66）。虽然在小肿瘤中肿瘤面积的测定与肿瘤质量具有良好的相关性（r = 0.89），但在大肿瘤中肿瘤面积与肿瘤质量的相关性较差（r = 0.41）。我们的结论是，通过三个轴的测量确定椭圆体体积始终可以最准确地估计相对和实际肿瘤质量。
The athymic (nude) mouse is a useful model for studying the biology and response to therapies of human tumors in vivo. A survey of recent literature revealed the use of 19 different formulas for determining the size of subcutaneous tumors grown as xenografts in nude mice (2 for determining tumor area, 3 for tumor diameter, and 14 for calculating tumor volume). We compared the volumes, areas, and diameters predicted by each of the 19 formulas with the actual weights of 50 tumors ranging from 0.46 to 22.0 g established in nude mice as xenografts from human cell lines. In addition to determining how well each formula predicted relative tumor size, we analyzed how well each formula estimated actual tumor mass. The ellipsoid volume formulas (.pi./6 .times. L .times. W .times. H and 1/2 .times. L .times. W .times. H) were best for estimating tumor mass (r = 0.93), whereas measurements of diameter correlated poorly with tumor mass (r < 0.66). Although determination of tumor area correlated well with tumor mass in small tumors (r = 0.89), correlations of area with tumor mass for large tumors were poor (r = 0.41). We conclude that determination of the ellipsoid volume from measurements of three axes consistently yields the most accurate estimations of both relative and actual tumor mass.